Anti-eIF2A antibody [3A7B11] (ab181467)
![Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-260193-anti-eif2a-antibody-3a7b11-western-blot.jpg "Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)")
Key features and details
- Mouse monoclonal [3A7B11] to eIF2A
- Suitable for: Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-eIF2A antibody [3A7B11]
See all eIF2A primary antibodies -
Description
Mouse monoclonal [3A7B11] to eIF2A -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human eIF2A aa 448-576. (Expressed in E.coli).
Sequence:PPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAA KQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAI EQLKEQAATGKQLEKNQLEK IQKETALLQ
Database link: Q9BY44 -
Positive control
- Recombinant Human eIF2A (aa 448-576) protein; eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate; MCF7, PC-12, HepG2, HeLa, Cos7, K562, Jurkat, A431 and NIH3T3 cell lysates; HepG2 cells; Human cervical cancer and bladder cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3A7B11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A-431 whole cell lysate (ab30132)
- NIH/3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- A-431 whole cell lysate (ab7909)
- K-562 whole cell lysate (ab7911)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P |
1/200 - 1/1000.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 65 kDa.
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| Notes |
|---|
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Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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IHC-P
1/200 - 1/1000. |
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WB
1/500 - 1/2000. Predicted molecular weight: 65 kDa. |
Target
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Function
Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. -
Tissue specificity
Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta. -
Sequence similarities
Belongs to the WD repeat EIF2A family.
Contains 3 WD repeats. - Information by UniProt
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Database links
- Entrez Gene: 83939 Human
- Entrez Gene: 229317 Mouse
- Entrez Gene: 502531 Rat
- Omim: 609234 Human
- SwissProt: Q9BY44 Human
- SwissProt: Q8BJW6 Mouse
- SwissProt: P68101 Rat
- Unigene: 655782 Human
see all -
Alternative names
- 65 kDa eukaryotic translation initiation factor 2A antibody
- CDA 02 antibody
- CDA02 antibody
see all
Images
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Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: eIF2A knockout HAP1 cell lysate (20 µg)
Lane 3: Ramos cell lysate (20 µg)
Lane 4: MOLT4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181467 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab181467 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab181467 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210608-anti-eif2a-antibody-3a7b11-western-blot.jpg)
Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : COS-7 cell lysate
Lane 6 : K562 cell lysate
Lane 7 : Jurkat cell lysate
Lane 8 : A431 cell lysate
Lane 9 : NIH3T3 cell lysate
Predicted band size: 65 kDa -
![Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210609-anti-eif2a-antibody-3a7b11-western-blot.jpg)
Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution + Recombinant Human eIF2A (aa 448-576) protein
Predicted band size: 65 kDaExpected MWt is 40.3 kDa.
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![Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210610-anti-eif2a-antibody-3a7b11-western-blot.jpg)
Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate
Lane 2 : eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 65 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210614-anti-eif2a-antibody-3a7b11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210613-anti-eif2a-antibody-3a7b11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.
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![Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467)](https://www.abcam.com/ps/products/181/ab181467/Images/ab181467-210612-anti-eif2a-antibody-3a7b11-flow-cytometry.jpg)
Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467)Flow cytometric analysis of HepG2 cells labeling eIF2A with ab181467 at 1/200 dilution (green) compared to a negative control (red).
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab181467 has been referenced in 1 publication.
- Barreiro E et al. Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases. J Cell Physiol 234:11315-11329 (2019). PubMed: 30565689
