Overview

  • Product name

    Anti-eIF2A antibody [3A7B11]
    See all eIF2A primary antibodies
  • Description

    Mouse monoclonal [3A7B11] to eIF2A
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human eIF2A aa 448-576. (Expressed in E.coli).
    Sequence:

    PPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAA KQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAI EQLKEQAATGKQLEKNQLEK IQKETALLQ


    Database link: Q9BY44

  • Positive control

    • Recombinant Human eIF2A (aa 448-576) protein; eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate; MCF7, PC-12, HepG2, HeLa, Cos7, K562, Jurkat, A431 and NIH3T3 cell lysates; HepG2 cells; Human cervical cancer and bladder cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab181467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
  • Tissue specificity

    Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
  • Sequence similarities

    Belongs to the WD repeat EIF2A family.
    Contains 3 WD repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 65 kDa eukaryotic translation initiation factor 2A antibody
    • CDA 02 antibody
    • CDA02 antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • eIF-2A antibody
    • EIF2 antibody
    • eif2a antibody
    • EIF2A_HUMAN antibody
    • Eukaryotic translation initiation factor 2A 65kDa antibody
    • Eukaryotic translation initiation factor 2A antibody
    • MST089 antibody
    • MSTP004 antibody
    • MSTP089 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: eIF2A knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: MOLT4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181467 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab181467 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab181467 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Cos7 cell lysate
    Lane 6 : K562 cell lysate
    Lane 7 : Jurkat cell lysate
    Lane 8 : A431 cell lysate
    Lane 9 : NIH3T3 cell lysate

    Predicted band size: 65 kDa

  • Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution + Recombinant Human eIF2A (aa 448-576) protein

    Predicted band size: 65 kDa



    Expected MWt is 40.3 kDa.

  • All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

    Lane 1 : Non-transfected HEK293 cell lysate
    Lane 2 : eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 65 kDa

  • Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

  • Flow cytometric analysis of HepG2 cells labeling eIF2A with ab181467 at 1/200 dilution (green) compared to a negative control (red).

References

ab181467 has not yet been referenced specifically in any publications.

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