Validated using a knockout cell line

Anti-eIF2A antibody [3A7B11] (ab181467)

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Key features and details

  • Mouse monoclonal [3A7B11] to eIF2A
  • Suitable for: Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-eIF2A antibody [3A7B11]
    See all eIF2A primary antibodies

  • Description

    Mouse monoclonal [3A7B11] to eIF2A

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey

  • Immunogen

    Recombinant fragment corresponding to Human eIF2A aa 448-576. (Expressed in E.coli).
    Sequence:

    PPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAA KQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAI EQLKEQAATGKQLEKNQLEK IQKETALLQ


    Database link: Q9BY44

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human eIF2A (aa 448-576) protein; eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate; MCF7, PC-12, HepG2, HeLa, Cos7, K562, Jurkat, A431 and NIH3T3 cell lysates; HepG2 cells; Human cervical cancer and bladder cancer tissues.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab181467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.

  • Tissue specificity

    Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.

  • Sequence similarities

    Belongs to the WD repeat EIF2A family.
    Contains 3 WD repeats.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 65 kDa eukaryotic translation initiation factor 2A antibody
    • CDA 02 antibody
    • CDA02 antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • eIF-2A antibody
    • EIF2 antibody
    • eif2a antibody
    • EIF2A_HUMAN antibody
    • Eukaryotic translation initiation factor 2A 65kDa antibody
    • Eukaryotic translation initiation factor 2A antibody
    • MST089 antibody
    • MSTP004 antibody
    • MSTP089 antibody
    see all

Images

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: eIF2A knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: MOLT4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181467 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab181467 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab181467 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
    All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : COS-7 cell lysate
    Lane 6 : K562 cell lysate
    Lane 7 : Jurkat cell lysate
    Lane 8 : A431 cell lysate
    Lane 9 : NIH3T3 cell lysate

    Predicted band size: 65 kDa

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
    Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution + Recombinant Human eIF2A (aa 448-576) protein

    Predicted band size: 65 kDa



    Expected MWt is 40.3 kDa.

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)
    All lanes : Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

    Lane 1 : Non-transfected HEK293 cell lysate
    Lane 2 : eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)

    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

  • Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467)
    Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467)

    Flow cytometric analysis of HepG2 cells labeling eIF2A with ab181467 at 1/200 dilution (green) compared to a negative control (red).

References (1)

Publishing research using ab181467? Please let us know so that we can cite the reference in this datasheet.

ab181467 has been referenced in 1 publication.

  • Barreiro E  et al. Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases. J Cell Physiol 234:11315-11329 (2019). PubMed: 30565689

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