Anti-eIF2A antibody (ab235147)
Key features and details
- Rabbit polyclonal to eIF2A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF2A antibody
See all eIF2A primary antibodies -
Description
Rabbit polyclonal to eIF2A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: eIF2A human protein over HEK-293 expressing lysate; Daudi and HeLa whole cell lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/1000. Predicted molecular weight: 65 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/1000. Predicted molecular weight: 65 kDa. |
Target
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Function
Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. -
Tissue specificity
Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta. -
Sequence similarities
Belongs to the WD repeat EIF2A family.
Contains 3 WD repeats. - Information by UniProt
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Database links
- Entrez Gene: 83939 Human
- Omim: 609234 Human
- SwissProt: Q9BY44 Human
- Unigene: 655782 Human
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Alternative names
- 65 kDa eukaryotic translation initiation factor 2A antibody
- CDA 02 antibody
- CDA02 antibody
see all
Images
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4% paraformaldehyde-fixed, 0.3%Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling elF2A using ab235147 at 5 µg/mL overnight at 4ºC in ICC/IF analysis. Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 µg/mL for 1 hour at room temperature.
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All lanes : Anti-eIF2A antibody (ab235147) at 5 µg/ml
Lane 1 : Daudi whole cell lysate at 35 µg
Lane 2 : MCF7 whole cell lysate at 35 µg
Lane 3 : HeLa whole cell lysate
Secondary
All lanes : Donkey anti-Goat HRP at 1/40000 dilution
Predicted band size: 65 kDa
Exposure time: 35 seconds -
All lanes : Anti-eIF2A antibody (ab235147) at 1/1000 dilution
Lane 1 : eIF2A human protein over HEK293 expressing lysate
Lane 2 : HEK293 lysate (empty vector)
Secondary
All lanes : Donkey Fab2 anti-Goat HRP at 1/70000 dilution
Predicted band size: 65 kDa
Exposure time: 15 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab235147 has been referenced in 1 publication.
- Wang X et al. GRP94 Inhabits the Immortalized Porcine Hepatic Stellate Cells Apoptosis under Endoplasmic Reticulum Stress through Modulating the Expression of IGF-1 and Ubiquitin. Int J Mol Sci 23:N/A (2022). PubMed: 36430538