Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: eIF2A human protein over HEK-293 expressing lysate; Daudi and HeLa whole cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
  • Tissue specificity

    Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
  • Sequence similarities

    Belongs to the WD repeat EIF2A family.
    Contains 3 WD repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 65 kDa eukaryotic translation initiation factor 2A antibody
    • CDA 02 antibody
    • CDA02 antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • eIF-2A antibody
    • EIF2 antibody
    • eif2a antibody
    • EIF2A_HUMAN antibody
    • Eukaryotic translation initiation factor 2A 65kDa antibody
    • Eukaryotic translation initiation factor 2A antibody
    • MST089 antibody
    • MSTP004 antibody
    • MSTP089 antibody
    see all

Images

  • 4% paraformaldehyde-fixed, 0.3%Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling elF2A using ab235147 at 5 µg/mL overnight at 4ºC in ICC/IF analysis. Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 µg/mL for 1 hour at room temperature.

  • All lanes : Anti-eIF2A antibody (ab235147) at 5 µg/ml

    Lane 1 : Daudi whole cell lysate at 35 µg
    Lane 2 : MCF7 whole cell lysate at 35 µg
    Lane 3 : HeLa whole cell lysate

    Secondary
    All lanes : Donkey anti-Goat HRP at 1/40000 dilution

    Predicted band size: 65 kDa


    Exposure time: 35 seconds
  • All lanes : Anti-eIF2A antibody (ab235147) at 1/1000 dilution

    Lane 1 : eIF2A human protein over HEK293 expressing lysate
    Lane 2 : HEK293 lysate (empty vector)

    Secondary
    All lanes : Donkey Fab2 anti-Goat HRP at 1/70000 dilution

    Predicted band size: 65 kDa


    Exposure time: 15 seconds

References

ab235147 has not yet been referenced specifically in any publications.

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