Key features and details
- Rabbit polyclonal to eIF2A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF2A antibody
See all eIF2A primary antibodies
DescriptionRabbit polyclonal to eIF2A
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: eIF2A human protein over HEK-293 expressing lysate; Daudi and HeLa whole cell lysate. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000. Predicted molecular weight: 65 kDa.|
FunctionFunctions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
Tissue specificityWidely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
Sequence similaritiesBelongs to the WD repeat EIF2A family.
Contains 3 WD repeats.
- Information by UniProt
- 65 kDa eukaryotic translation initiation factor 2A antibody
- CDA 02 antibody
- CDA02 antibody
4% paraformaldehyde-fixed, 0.3%Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling elF2A using ab235147 at 5 µg/mL overnight at 4ºC in ICC/IF analysis. Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 µg/mL for 1 hour at room temperature.
All lanes : Anti-eIF2A antibody (ab235147) at 5 µg/ml
Lane 1 : Daudi whole cell lysate at 35 µg
Lane 2 : MCF7 whole cell lysate at 35 µg
Lane 3 : HeLa whole cell lysate
All lanes : Donkey anti-Goat HRP at 1/40000 dilution
Predicted band size: 65 kDa
Exposure time: 35 seconds
All lanes : Anti-eIF2A antibody (ab235147) at 1/1000 dilution
Lane 1 : eIF2A human protein over HEK293 expressing lysate
Lane 2 : HEK293 lysate (empty vector)
All lanes : Donkey Fab2 anti-Goat HRP at 1/70000 dilution
Predicted band size: 65 kDa
Exposure time: 15 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235147 has not yet been referenced specifically in any publications.