Product nameAnti-eIF2A antibody
See all eIF2A primary antibodies
DescriptionRabbit polyclonal to eIF2A
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human eIF2A aa 525-575. The exact sequence is proprietary.
Database link: Q9BY44
- IHC-P: Human Breast Carcinoma tissue. IP: HeLa whole cell lysate. WB: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab264254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||1/100 - 1/500.|
|WB||1/2000 - 1/10000.|
FunctionFunctions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
Tissue specificityWidely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
Sequence similaritiesBelongs to the WD repeat EIF2A family.
Contains 3 WD repeats.
- Information by UniProt
- 65 kDa eukaryotic translation initiation factor 2A antibody
- CDA 02 antibody
- CDA02 antibody
All lanes : Anti-eIF2A antibody (ab264254) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Exposure time: 10 seconds
Immunohistochemical analysis of FFPE human breast carcinoma tissue section labelling eIF2A with ab264254 at 1/200 dilution. DAB was used a counterstain.
eIF2A was immunoprecipitated from 1mg of HeLa whole cell lysates using eIF2A antibodies at 3μg/reaction. Western blot was performed on the immunprecipitates using ab264253 (lane 1), ab264254 (lane 2) and a control IgG (lane 3) at 1μg/ml.
Detected with chemilimuniscence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264254 has not yet been referenced specifically in any publications.