Overview

  • Product name

    Anti-eIF2A antibody [EPR11041]
    See all eIF2A primary antibodies
  • Description

    Rabbit monoclonal [EPR11041] to eIF2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human eIF2A. The exact sequence is proprietary.

  • Positive control

    • Ramos, MOLT4, U87-MG and Raji cell lysates; Raji cells. ICC/IF: HAP1 wildtype and HAP1-EIF2A knockout cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC/IF Use a concentration of 1 µg/ml.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
    • Tissue specificity

      Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
    • Sequence similarities

      Belongs to the WD repeat EIF2A family.
      Contains 3 WD repeats.
    • Information by UniProt
    • Database links

    • Alternative names

      • 65 kDa eukaryotic translation initiation factor 2A antibody
      • CDA 02 antibody
      • CDA02 antibody
      • EIF 2 antibody
      • EIF 2A antibody
      • eIF-2A antibody
      • EIF2 antibody
      • eif2a antibody
      • EIF2A_HUMAN antibody
      • Eukaryotic translation initiation factor 2A 65kDa antibody
      • Eukaryotic translation initiation factor 2A antibody
      • MST089 antibody
      • MSTP004 antibody
      • MSTP089 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: eIF2A knockout HAP1 cell lysate (20 µg)
      Lane 3: U87-MG cell lysate (20 µg)
      Lane 4: MOLT4 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab157478 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab157478 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab157478 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • ab157478 staining eIF2A in wild-type HAP1 cells (top panel) and EIF2A knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab157478 at 1μg/ml and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

       

    • All lanes : Anti-eIF2A antibody [EPR11041] (ab157478) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 65 kDa

    • Immunofluorescent analysis of Raji cells labeling eIF2A with ab157478 at 1/100 dilution.

    References

    ab157478 has not yet been referenced specifically in any publications.

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