Overview

  • Product name

    Anti-eIF2A antibody [EPR11042] (HRP)
    See all eIF2A primary antibodies
  • Description

    Rabbit monoclonal [EPR11042] to eIF2A (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human eIF2A aa 450-550. The exact sequence is proprietary.
    Database link: Q9BY44

  • Positive control

    • MOLT4, Ramos, HAP1 WT (shows pos.) and HAP1 eIF2A KO (shows neg.)
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 65 kDa.

Target

  • Function

    Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
  • Tissue specificity

    Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
  • Sequence similarities

    Belongs to the WD repeat EIF2A family.
    Contains 3 WD repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • 65 kDa eukaryotic translation initiation factor 2A antibody
    • CDA 02 antibody
    • CDA02 antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • eIF-2A antibody
    • EIF2 antibody
    • eif2a antibody
    • EIF2A_HUMAN antibody
    • Eukaryotic translation initiation factor 2A 65kDa antibody
    • Eukaryotic translation initiation factor 2A antibody
    • MST089 antibody
    • MSTP004 antibody
    • MSTP089 antibody
    see all

Images

  • All lanes : Anti-eIF2A antibody [EPR11042] (HRP) (ab215342) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : EIF2A (eIF2A) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa


    Exposure time: 8 minutes


    ab215342 was shown to specifically react with eIF2A in wild-type HAP1 cells as signal was lost in EIF2A (eIF2A) knockout cells. Wild-type and EIF2A (eIF2A) knockout samples were subjected to SDS-PAGE. ab215342 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • All lanes : Anti-eIF2A antibody [EPR11042] (HRP) (ab215342) at 1/5000 dilution

    Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : HAP1 WT at 20 µg
    Lane 4 : eIF2A knockout HAP1 at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215342 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab215342 has not yet been referenced specifically in any publications.

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