Recombinant
RabMAb

Anti-eIF2B epsilon antibody [EPR13533] (ab181033)

Overview

  • Product name
    Anti-eIF2B epsilon antibody [EPR13533]
    See all eIF2B epsilon primary antibodies
  • Description
    Rabbit monoclonal [EPR13533] to eIF2B epsilon
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human eIF2B epsilon aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13144

  • Positive control
    • Hela and Jurkat cell lysates. Human breast and uterus tissue. Hela cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.

Target

  • Function
    Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
  • Involvement in disease
    Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
  • Sequence similarities
    Belongs to the eIF-2B gamma/epsilon subunits family.
    Contains 1 W2 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CACH antibody
    • CLE antibody
    • EI2BE_HUMAN antibody
    • EIF 2B antibody
    • eIF 2B GDP GTP exchange factor antibody
    • eIF 2B GDP GTP exchange factor subunit epsilon antibody
    • eIF-2B GDP-GTP exchange factor subunit epsilon antibody
    • EIF2B antibody
    • EIF2B5 antibody
    • EIF2BE antibody
    • EIF2Bepsilon antibody
    • Eukaryotic Translation Initiation Factor 2 Beta Epsilon antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon antibody
    • LVWM antibody
    • Translation initiation factor eIF 2B subunit epsilon antibody
    • Translation initiation factor eIF-2B subunit epsilon antibody
    see all

Images

  • All lanes : Anti-eIF2B epsilon antibody [EPR13533] (ab181033) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 80 kDa

  • Immunofluorescent staining of HeLa cells labeling eIF2B epsilon with ab181033 at 1/100 dilution. Counterstain DAPI.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling eIF2B epsilon with ab181033 at 1/50 dilution.

  •  Immunohistochemical analysis of paraffin-embedded human breast tissue labeling eiF2B epsilon with ab181033 at 1/50 dilution.

References

ab181033 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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