Anti-eIF2B epsilon antibody - N-terminal (ab171393)

Overview

  • Product name
    Anti-eIF2B epsilon antibody - N-terminal
    See all eIF2B epsilon primary antibodies
  • Description
    Rabbit polyclonal to eIF2B epsilon - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human eIF2B epsilon aa 35-65 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q13144

  • Positive control
    • Mouse testis tissue lysate, Human breast tissue, U251 cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab171393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 80 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
  • Involvement in disease
    Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
  • Sequence similarities
    Belongs to the eIF-2B gamma/epsilon subunits family.
    Contains 1 W2 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CACH antibody
    • CLE antibody
    • EI2BE_HUMAN antibody
    • EIF 2B antibody
    • eIF 2B GDP GTP exchange factor antibody
    • eIF 2B GDP GTP exchange factor subunit epsilon antibody
    • eIF-2B GDP-GTP exchange factor subunit epsilon antibody
    • EIF2B antibody
    • EIF2B5 antibody
    • EIF2BE antibody
    • EIF2Bepsilon antibody
    • Eukaryotic Translation Initiation Factor 2 Beta Epsilon antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon antibody
    • LVWM antibody
    • Translation initiation factor eIF 2B subunit epsilon antibody
    • Translation initiation factor eIF-2B subunit epsilon antibody
    see all

Images

  • Anti-eIF2B epsilon antibody - N-terminal (ab171393) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

    Predicted band size: 80 kDa

  • Anti-eIF2B epsilon antibody - N-terminal (ab171393) at 1/100 dilution + U251 cell line lysate at 35 µg

    Predicted band size: 80 kDa

  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human breast tissue labeling eIF2B epsilon with ab171393 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171393 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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