Anti-EIF2B1 antibody (ab229704)
Key features and details
- Rabbit polyclonal to EIF2B1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EIF2B1 antibody
See all EIF2B1 primary antibodies -
Description
Rabbit polyclonal to EIF2B1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human EIF2B1 aa 1 to the C-terminus.
Database link: Q14232 -
Positive control
- WB: MCF7, K562, Jurkat and A431 whole cell lysates; Mouse liver and brain tissue lysates. IHC-P: Human prostate cancer and breast cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- Mouse liver tissue lysate - total protein (ab29301)
- MCF7 whole cell lysate (ab29537)
- Jurkat whole cell lysate (ab30128)
- A-431 whole cell lysate (ab30132)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- Jurkat whole cell lysate (ab7899)
- A-431 whole cell lysate (ab7909)
- K-562 whole cell lysate (ab7911)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 34 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 34 kDa. |
Target
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Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
Involvement in disease
Defects in EIF2B1 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. -
Sequence similarities
Belongs to the eIF-2B alpha/beta/delta subunits family. - Information by UniProt
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Database links
- Entrez Gene: 516670 Cow
- Entrez Gene: 101926282 Cynomolgus monkey
- Entrez Gene: 1967 Human
- Entrez Gene: 209354 Mouse
- Entrez Gene: 100172592 Orangutan
- Entrez Gene: 64514 Rat
- Omim: 606686 Human
- SwissProt: Q0IIF2 Cow
see all -
Alternative names
- EI2BA_HUMAN antibody
- eIF-2B GDP-GTP exchange factor subunit alpha antibody
- EIF2B antibody
see all
Images
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All lanes : Anti-EIF2B1 antibody (ab229704) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
Paraffin-embedded human prostate cancer tissue stained for EIF2B1 using ab229704 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human breast cancer tissue stained for EIF2B1 using ab229704 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229704 has not yet been referenced specifically in any publications.