Product nameAnti-eIF2B1 antibody [EPR13894(B)]
DescriptionRabbit monoclonal [EPR13894(B)] to eIF2B1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF2B1 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q14232
- SH-SH5Y, Jurkat and MCF7 cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).|
|IP||1/20 - 1/40.|
RelevanceeIF2B1 is one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
Cellular localizationPlasma membrane
- 26kDa antibody
- D5Ertd406e antibody
- EI2BA antibody
All lanes : Anti-eIF2B1 antibody [EPR13894(B)] (ab181186) at 1/10000 dilution
Lane 1 : SH-SH5Y cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation. ab181186 at 1/1000 labeling elF2B1 immunoprecipitated from MCF7 cell lysate using ab181186 at 1/50.
ab181186 has not yet been referenced specifically in any publications.