Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF2B3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 217-266 (ENGSITSIRS ELIPYLVRKQ FSSASSQQGQ EEKEEDLKKK ELKSLDIYSF) of Human eIF2B3.

  • Positive control

    • Human fetal liver lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • Function

    Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
  • Involvement in disease

    Defects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
  • Sequence similarities

    Belongs to the eIF-2B gamma/epsilon subunits family.
  • Information by UniProt
  • Database links

  • Form

    It localizes to the cytosol.
  • Alternative names

    • EI2BG_HUMAN antibody
    • EIF 2B antibody
    • eIF 2B GDP GTP exchange factor subunit gamma antibody
    • eIF-2B GDP-GTP exchange factor subunit gamma antibody
    • eIF2B-gamma antibody
    • Eif2b3 antibody
    • EIF2Bgamma antibody
    • Eukaryotic translation initiation factor 2B subunit 3 gamma antibody
    • Eukaryotic translation initiation factor 2B, gamma antibody
    • eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa antibody
    • OTTHUMP00000010262 antibody
    • OTTHUMP00000010263 antibody
    • Translation initiation factor eIF-2B subunit gamma antibody
    see all

Images

  • Anti-eIF2B3 antibody (ab135351) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Predicted band size: 50 kDa

References

ab135351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (keratinocyte)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
keratinocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 25 2016

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