Anti-eIF2B4 antibody (ab104941)
Key features and details
- Goat polyclonal to eIF2B4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF2B4 antibody
See all eIF2B4 primary antibodies -
Description
Goat polyclonal to eIF2B4 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog -
Immunogen
Synthetic peptide:
C-RKDYGSKVSLFSH
, corresponding to internal sequence amino acids 190-202 of Human eIF2B4 (NP_751945.2; NP_001029288.1; NP_056451.3). -
Positive control
- K562 lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
Involvement in disease
Leukodystrophy with vanishing white matter -
Sequence similarities
Belongs to the eIF-2B alpha/beta/delta subunits family. - Information by UniProt
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Database links
- Entrez Gene: 8890 Human
- Entrez Gene: 13667 Mouse
- Entrez Gene: 117019 Rat
- Omim: 606687 Human
- SwissProt: Q3T058 Cow
- SwissProt: Q9UI10 Human
- SwissProt: Q61749 Mouse
- SwissProt: P41111 Rabbit
see all -
Alternative names
- EI2BD_HUMAN antibody
- EIF 2B antibody
- eIF 2B GDP GTP exchange factor subunit delta antibody
see all
Images
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Anti-eIF2B4 antibody (ab104941) at 0.3 µg/ml + K562 lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Datasheets and documents
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Datasheet download
References (0)
ab104941 has not yet been referenced specifically in any publications.