Key features and details
- Goat polyclonal to eIF2B4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF2B4 antibody
See all eIF2B4 primary antibodies
DescriptionGoat polyclonal to eIF2B4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog
- K562 lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab104941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
1 hour primary incubation is recommended for this product.
FunctionCatalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Involvement in diseaseLeukodystrophy with vanishing white matter
Sequence similaritiesBelongs to the eIF-2B alpha/beta/delta subunits family.
- Information by UniProt
- EI2BD_HUMAN antibody
- EIF 2B antibody
- eIF 2B GDP GTP exchange factor subunit delta antibody
Anti-eIF2B4 antibody (ab104941) at 0.3 µg/ml + K562 lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab104941 has not yet been referenced specifically in any publications.