Anti-eIF2B4 antibody (ab241573)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-eIF2B4 antibody
See all eIF2B4 primary antibodies -
Description
Rabbit polyclonal to eIF2B4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human eIF2B4 aa 50-100. The exact sequence is proprietary. (NP_001029288.1).
Database link: Q9UI10 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma and breast carcinoma tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241573 was affinity purified using an epitope specific to eIF2B delta/EIF2B4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
Target
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Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
Involvement in disease
Leukodystrophy with vanishing white matter -
Sequence similarities
Belongs to the eIF-2B alpha/beta/delta subunits family. - Information by UniProt
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Database links
- Entrez Gene: 8890 Human
- Omim: 606687 Human
- SwissProt: Q9UI10 Human
- Unigene: 169474 Human
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Alternative names
- EI2BD_HUMAN antibody
- EIF 2B antibody
- eIF 2B GDP GTP exchange factor subunit delta antibody
see all
Images
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Western blot - Anti-eIF2B4 antibody (ab241573)All lanes : Anti-eIF2B4 antibody (ab241573) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-eIF2B4 antibody (ab241573)eIF2B4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241573 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241573 at 0.4 µg/ml.
Lane 1: ab241573 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
Datasheets and documents
References
ab241573 has not yet been referenced specifically in any publications.
