Anti-eIF2B4 antibody (ab241573)
Key features and details
- Rabbit polyclonal to eIF2B4
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF2B4 antibody
See all eIF2B4 primary antibodies -
Description
Rabbit polyclonal to eIF2B4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human eIF2B4 aa 50-100. The exact sequence is proprietary. (NP_001029288.1).
Database link: Q9UI10 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma and breast carcinoma tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241573 was affinity purified using an epitope specific to eIF2B delta/EIF2B4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
Target
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Function
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. -
Involvement in disease
Leukodystrophy with vanishing white matter -
Sequence similarities
Belongs to the eIF-2B alpha/beta/delta subunits family. - Information by UniProt
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Database links
- Entrez Gene: 8890 Human
- Omim: 606687 Human
- SwissProt: Q9UI10 Human
- Unigene: 169474 Human
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Alternative names
- EI2BD_HUMAN antibody
- EIF 2B antibody
- eIF 2B GDP GTP exchange factor subunit delta antibody
see all
Images
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Western blot - Anti-eIF2B4 antibody (ab241573)All lanes : Anti-eIF2B4 antibody (ab241573) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-eIF2B4 antibody (ab241573)eIF2B4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241573 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241573 at 0.4 µg/ml.
Lane 1: ab241573 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241573 has not yet been referenced specifically in any publications.
