Key features and details
- Rabbit polyclonal to eIF2B4
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF2B4 antibody
See all eIF2B4 primary antibodies
DescriptionRabbit polyclonal to eIF2B4
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human eIF2B4 aa 50-100. The exact sequence is proprietary. (NP_001029288.1).
Database link: Q9UI10
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma and breast carcinoma tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241573 was affinity purified using an epitope specific to eIF2B delta/EIF2B4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 57 kDa.|
FunctionCatalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Involvement in diseaseLeukodystrophy with vanishing white matter
Sequence similaritiesBelongs to the eIF-2B alpha/beta/delta subunits family.
- Information by UniProt
- EI2BD_HUMAN antibody
- EIF 2B antibody
- eIF 2B GDP GTP exchange factor subunit delta antibody
All lanes : Anti-eIF2B4 antibody (ab241573) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds
eIF2B4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241573 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241573 at 0.4 µg/ml.
Lane 1: ab241573 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241573 has not yet been referenced specifically in any publications.