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    eif2b4-antibody-ab241573.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-eIF2B4 antibody (ab241573)

  • Datasheet
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Western blot - Anti-eIF2B4 antibody (ab241573)
  • Immunoprecipitation - Anti-eIF2B4 antibody (ab241573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)

Key features and details

  • Rabbit polyclonal to eIF2B4
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Protein
Recombinant Human eIF2B4 protein (ab127076)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-eIF2B4 antibody
    See all eIF2B4 primary antibodies
  • Description

    Rabbit polyclonal to eIF2B4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human eIF2B4 aa 50-100. The exact sequence is proprietary. (NP_001029288.1).
    Database link: Q9UI10

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma and breast carcinoma tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab241573 was affinity purified using an epitope specific to eIF2B delta/EIF2B4 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human eIF2B4 protein (ab127076)

Applications

Our Abpromise guarantee covers the use of ab241573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.

Target

  • Function

    Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
  • Involvement in disease

    Leukodystrophy with vanishing white matter
  • Sequence similarities

    Belongs to the eIF-2B alpha/beta/delta subunits family.
  • Target information above from: UniProt accession Q9UI10 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8890 Human
    • Omim: 606687 Human
    • SwissProt: Q9UI10 Human
    • Unigene: 169474 Human
    • Alternative names

      • EI2BD_HUMAN antibody
      • EIF 2B antibody
      • eIF 2B GDP GTP exchange factor subunit delta antibody
      • eIF-2B GDP-GTP exchange factor subunit delta antibody
      • Eif2b4 antibody
      • EIF2Bdelta antibody
      • eukaryotic translation initiation factor 2B subunit 4 antibody
      • Translation initiation factor eIF-2B subunit delta antibody
      see all

    Images

    • Western blot - Anti-eIF2B4 antibody (ab241573)
      Western blot - Anti-eIF2B4 antibody (ab241573)
      All lanes : Anti-eIF2B4 antibody (ab241573) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 57 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-eIF2B4 antibody (ab241573)
      Immunoprecipitation - Anti-eIF2B4 antibody (ab241573)

      eIF2B4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241573 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241573 at 0.4 µg/ml.

      Lane 1: ab241573 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 10 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)

      Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2B4 antibody (ab241573)

      Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for eIF2B4 using ab241573 at 1/200 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241573? Please let us know so that we can cite the reference in this datasheet.

    ab241573 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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