Overview

  • Product name

  • Description

    Rabbit polyclonal to EIF2D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 350 and 400 of Human EIF2D (NP_008824.2).

  • Positive control

    • HeLa and 293T whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab108218 was affinity purified using an epitope specific to EIF2D immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Trafficking receptor for phosphoglycoproteins. Localizes phosphoglycoproteins within endosomes and at the cell periphery where they participate in specific metabolic processes as well as intercellular adhesion.
  • Sequence similarities

    Belongs to the ligatin family.
    Contains 1 PUA domain.
    Contains 1 SUI1 domain.
  • Developmental stage

    Found during embryonic development and in early differentiated states.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Eukaryotic translation initiation factor 2D antibody
    • HCA56 antibody
    • Hepatocellular carcinoma associated antigen 56 antibody
    • Hepatocellular carcinoma-associated antigen 56 antibody
    • LGTN antibody
    • LIGA_HUMAN antibody
    • Ligatin antibody
    • OTTHUMP00000034537 antibody
    • OTTHUMP00000034538 antibody
    see all

Images

  • All lanes : Anti-EIF2D antibody (ab108218) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 65 kDa


    Exposure time: 3 minutes
  • ab108218 at 1 µg/ml detecting LGTN in HeLa whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an downstream epitope of LGTN
    Lane 2: ab108218 at 6 µg/mg of lysate
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 30 seconds

References

This product has been referenced in:

  • Götte B  et al. Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery. PLoS Pathog 15:e1007842 (2019). Read more (PubMed: 31199850) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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