Product nameAnti-EIF2D antibody
See all EIF2D primary antibodies
DescriptionRabbit polyclonal to EIF2D
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide corresponding to a region between residue 350 and 400 of Human EIF2D (NP_008824.2).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab108218 was affinity purified using an epitope specific to EIF2D immobilized on solid support.
Our Abpromise guarantee covers the use of ab108218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 65 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionTrafficking receptor for phosphoglycoproteins. Localizes phosphoglycoproteins within endosomes and at the cell periphery where they participate in specific metabolic processes as well as intercellular adhesion.
Sequence similaritiesBelongs to the ligatin family.
Contains 1 PUA domain.
Contains 1 SUI1 domain.
Developmental stageFound during embryonic development and in early differentiated states.
- Information by UniProt
- Eukaryotic translation initiation factor 2D antibody
- HCA56 antibody
- Hepatocellular carcinoma associated antigen 56 antibody
All lanes : Anti-EIF2D antibody (ab108218) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 3 minutes
ab108218 at 1 µg/ml detecting LGTN in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an downstream epitope of LGTN
Lane 2: ab108218 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds