Anti-EIF2G/EIF2S3 antibody (ab33207)
Key features and details
- Rabbit polyclonal to EIF2G/EIF2S3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-EIF2G/EIF2S3 antibody
See all EIF2G/EIF2S3 primary antibodies -
Description
Rabbit polyclonal to EIF2G/EIF2S3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
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Positive control
- WB: NIH 3T3, PC12 and MEF1 whole cell lysates, mouse liver, spinal cord, testis, ovary, heart and kidney tissue lysates. ICC/IF: MEF1 cells.
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General notes
This product was previously labelled as EIF2G
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab33207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EIF2G subfamily. - Information by UniProt
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Database links
- Entrez Gene: 1968 Human
- Entrez Gene: 26905 Mouse
- Entrez Gene: 26908 Mouse
- Entrez Gene: 100312984 Rat
- Entrez Gene: 299027 Rat
- Omim: 300161 Human
- SwissProt: P41091 Human
- SwissProt: Q9Z0N1 Mouse
see all -
Alternative names
- eIF-2-gamma X antibody
- eIF-2gA antibody
- eIF-2gX antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EIF2G/EIF2S3 antibody (ab33207)
ICC/IF image of ab33207 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33207, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml
Lane 1 : Mouse Embryonic Fibroblast 1
Lane 2 :NIH 3T3 whole cell lysate (ab7179)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab33207 also gave a positive result in mouse adult brain, testes, heart, skeletal muscle, brain (0 day) and fetal (14 day) lysates. -
Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : Heart (Mouse) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 7 : Spinal Cord (Mouse) Tissue Lysate
Lane 8 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 9 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Protocols
Datasheets and documents
References (1)
ab33207 has been referenced in 1 publication.
- de Almeida RA et al. A yeast purification system for human translation initiation factors eIF2 and eIF2Be and their use in the diagnosis of CACH/VWM disease. PLoS One 8:e53958 (2013). WB ; Human . PubMed: 23335982
