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    eif2geif2s3-antibody-ab33207.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-EIF2G/EIF2S3 antibody (ab33207)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-EIF2G/EIF2S3 antibody (ab33207)
  • Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)
  • Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)

Key features and details

  • Rabbit polyclonal to EIF2G/EIF2S3
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-EIF2G/EIF2S3 antibody
    See all EIF2G/EIF2S3 primary antibodies
  • Description

    Rabbit polyclonal to EIF2G/EIF2S3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse EIF2G/EIF2S3 (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab33374, ab33375)

  • Positive control

    • WB: NIH 3T3, PC12 and MEF1 whole cell lysates, mouse liver, spinal cord, testis, ovary, heart and kidney tissue lysates. ICC/IF: MEF1 cells.
  • General notes

     This product was previously labelled as EIF2G

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab33207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EIF2G subfamily.
  • Target information above from: UniProt accession P41091 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1968 Human
    • Entrez Gene: 26905 Mouse
    • Entrez Gene: 26908 Mouse
    • Entrez Gene: 100312984 Rat
    • Entrez Gene: 299027 Rat
    • Omim: 300161 Human
    • SwissProt: P41091 Human
    • SwissProt: Q9Z0N1 Mouse
    • SwissProt: P81795 Rat
    • Unigene: 539684 Human
    • Unigene: 218851 Mouse
    • Unigene: 250909 Mouse
    • Unigene: 220237 Rat
    • Unigene: 87025 Rat
    see all
  • Alternative names

    • eIF-2-gamma X antibody
    • eIF-2gA antibody
    • eIF-2gX antibody
    • EIF2 antibody
    • EIF2G antibody
    • EIF2gamma antibody
    • EIF2S3 antibody
    • Eukaryotic translation initiation factor 2 gamma antibody
    • Eukaryotic translation initiation factor 2 gamma subunit antibody
    • Eukaryotic translation initiation factor 2 subunit 3 antibody
    • Eukaryotic translation initiation factor 2 subunit 3 gamma 52kDa antibody
    • Eukaryotic translation initiation factor 2 subunit gamma X antibody
    • IF2G_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EIF2G/EIF2S3 antibody (ab33207)
    Immunocytochemistry/ Immunofluorescence - Anti-EIF2G/EIF2S3 antibody (ab33207)

    ICC/IF image of ab33207 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33207, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)
    Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)
    All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml

    Lane 1 : Mouse Embryonic Fibroblast 1
    Lane 2 : NIH 3T3 whole cell lysate (ab7179)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    ab33207 also gave a positive result in mouse adult brain, testes, heart, skeletal muscle, brain (0 day) and fetal (14 day) lysates.
  • Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)
    Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)
    All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Heart (Mouse) Tissue Lysate
    Lane 5 : Kidney (Mouse) Tissue Lysate
    Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 7 : Spinal Cord (Mouse) Tissue Lysate
    Lane 8 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 9 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab33207? Please let us know so that we can cite the reference in this datasheet.

    ab33207 has been referenced in 1 publication.

    • de Almeida RA  et al. A yeast purification system for human translation initiation factors eIF2 and eIF2Be and their use in the diagnosis of CACH/VWM disease. PLoS One 8:e53958 (2013). WB ; Human . PubMed: 23335982

    Customer reviews and Q&As

    Show All Reviews Q&A
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