Anti-EIF2G/EIF2S3 antibody (ab33207)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-EIF2G/EIF2S3 antibody
See all EIF2G/EIF2S3 primary antibodies -
Description
Rabbit polyclonal to EIF2G/EIF2S3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
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Positive control
- WB: NIH 3T3, PC12 and MEF1 whole cell lysates, mouse liver, spinal cord, testis, ovary, heart and kidney tissue lysates. ICC/IF: MEF1 cells.
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General notes
This product was previously labelled as EIF2G
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab33207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EIF2G subfamily. - Information by UniProt
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Database links
- Entrez Gene: 1968 Human
- Entrez Gene: 26905 Mouse
- Entrez Gene: 26908 Mouse
- Entrez Gene: 100312984 Rat
- Entrez Gene: 299027 Rat
- Omim: 300161 Human
- SwissProt: P41091 Human
- SwissProt: Q9Z0N1 Mouse
see all -
Alternative names
- eIF-2-gamma X antibody
- eIF-2gA antibody
- eIF-2gX antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EIF2G/EIF2S3 antibody (ab33207)ICC/IF image of ab33207 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33207, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml
Lane 1 : Mouse Embryonic Fibroblast 1
Lane 2 :NIH 3T3 whole cell lysate (ab7179)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab33207 also gave a positive result in mouse adult brain, testes, heart, skeletal muscle, brain (0 day) and fetal (14 day) lysates. -
Western blot - Anti-EIF2G/EIF2S3 antibody (ab33207)All lanes : Anti-EIF2G/EIF2S3 antibody (ab33207) at 1 µg/ml
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : Heart (Mouse) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 7 : Spinal Cord (Mouse) Tissue Lysate
Lane 8 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 9 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Datasheets and documents
References
This product has been referenced in:
- de Almeida RA et al. A yeast purification system for human translation initiation factors eIF2 and eIF2Be and their use in the diagnosis of CACH/VWM disease. PLoS One 8:e53958 (2013). WB ; Human . Read more (PubMed: 23335982) »
