Overview

  • Product name

    Anti-EIF2G/EIF2S3 antibody
    See all EIF2G/EIF2S3 primary antibodies
  • Description

    Rabbit polyclonal to EIF2G/EIF2S3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse EIF2G/EIF2S3 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab33375)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: MEF1, NIH3T3
  • General notes

     This product was previously labelled as EIF2G

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EIF2G subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • eIF-2-gamma X antibody
    • eIF-2gA antibody
    • eIF-2gX antibody
    • EIF2 antibody
    • EIF2G antibody
    • EIF2gamma antibody
    • EIF2S3 antibody
    • Eukaryotic translation initiation factor 2 gamma antibody
    • Eukaryotic translation initiation factor 2 gamma subunit antibody
    • Eukaryotic translation initiation factor 2 subunit 3 antibody
    • Eukaryotic translation initiation factor 2 subunit 3 gamma 52kDa antibody
    • Eukaryotic translation initiation factor 2 subunit gamma X antibody
    • IF2G_HUMAN antibody
    see all

Images

  • All lanes : Anti-EIF2G/EIF2S3 antibody (ab33376) at 1/250 dilution

    Lane 1 : Mouse Embryonic Fibroblast 1
    Lane 2 : NIH 3T3 whole cell lysate (ab7179)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    ab33376 also gave a positive result in mouse adult brain, testes, heart, skeletal muscle, brain (0 day) and fetal (14 day) lysates.

References

ab33376 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab33376.
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