Product nameAnti-EIF2G/EIF2S3 antibody
See all EIF2G/EIF2S3 primary antibodies
DescriptionRabbit polyclonal to EIF2G/EIF2S3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide corresponding to Mouse EIF2G/EIF2S3 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: MEF1, NIH3T3
This product was previously labelled as EIF2G
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab33376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
FunctioneIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EIF2G subfamily.
- Information by UniProt
- eIF-2-gamma X antibody
- eIF-2gA antibody
- eIF-2gX antibody
All lanes : Anti-EIF2G/EIF2S3 antibody (ab33376) at 1/250 dilution
Lane 1 : Mouse Embryonic Fibroblast 1
Lane 2 :
NIH 3T3 whole cell lysate (ab7179)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab33376 also gave a positive result in mouse adult brain, testes, heart, skeletal muscle, brain (0 day) and fetal (14 day) lysates.
ab33376 has not yet been referenced specifically in any publications.