Overview

  • Product name

  • Description

    Rabbit polyclonal to EIF2S1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to a region of Rat EIF2S1.

  • Positive control

    • Mouse 3T3 cell lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

Applications

Our Abpromise guarantee covers the use of ab115822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
ICC Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities

    Belongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • Post-translational
    modifications

    Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • Cellular localization

    Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • EIF 2 alpha antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • EIF 2alpha antibody
    • eIF-2-alpha antibody
    • eIF-2A antibody
    • EIF-2alpha antibody
    • EIF2 alpha antibody
    • EIF2 antibody
    • EIF2A antibody
    • EIF2S1 antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    • IF2A_HUMAN antibody
    see all

Images

  • ICC/IF image of ab115822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115822 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-EIF2S1 antibody (ab115822) at 1 µg/ml + Mouse 3T3 cell lysate

    Developed using the ECL technique.

    Predicted band size: 65 kDa

References

ab115822 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you very much for your interest in Abcam antibodies.

For most of the antibodies we have in our catalog it is possible to know the immunogen sequence or at least the immunogenic region and compare it with the sequence of interest.

For example, we know that the anti-caveolin ab18199 shows 76% homology with the chicken protein (SwissProt P35431).

If you want to test this antibody for exampleI can offer a discount off a future purchase if you buy ab18199 now, test it inChicken and submit feedback to us in the form of an Abreview.It doesn’t matter whether theAbreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test an Abcam antibody in Chicken. I will then send a discount code. This code must be issued before purchasing the antibody you would like to test, so please wait for my reply before ordering.

2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

3. Test it in in Chicken.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for Chicken, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

If you are interested in this offer, please inform us which application you would like the antibody in so I can narrow down the choice of the antibodies.

I am going to contact the lab where the anti-eIF2 alpha(ab115822) is produced in order to ask for the immunogen sequence or region.

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