Anti-EIF2S1 antibody (ab210077)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-EIF2S1 antibody
See all EIF2S1 primary antibodies -
Description
Rabbit polyclonal to EIF2S1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Zebrafish
Predicted to work with: Mouse, Rat, Cow, Human
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Immunogen
Recombinant fragment within Zebrafish EIF2S1 aa 2-315. The exact sequence is proprietary.
Database link: 321564 -
Positive control
- Whole zebrafish and zebrafish eye whole cell lysates; zebrafish kidney tube tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab210077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 36 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
Sequence similarities
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain. -
Post-translational
modificationsSubstrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. -
Cellular localization
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 327694 Cow
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Entrez Gene: 54318 Rat
- Entrez Gene: 321564 Zebrafish
- Omim: 603907 Human
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
see all -
Alternative names
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
Images
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Western blot - Anti-EIF2S1 antibody (ab210077)All lanes : Anti-EIF2S1 antibody (ab210077) at 1/1000 dilution
Lane 1 : whole zebrafish whole cell lysate
Lane 2 : zebrafish eye whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody (ab210077)Immunohistochemical analysis of paraffin-embedded zebrafish kidney tube tissue labeling EIF2S1 with ab210077 at 1/300 dilution.
References
ab210077 has not yet been referenced specifically in any publications.
