Anti-EIF2S1 antibody (ab26197)
Key features and details
- Rabbit polyclonal to EIF2S1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human, Drosophila melanogaster
- Isotype: IgG
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Overview
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Product name
Anti-EIF2S1 antibody
See all EIF2S1 primary antibodies -
Description
Rabbit polyclonal to EIF2S1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Drosophila melanogaster
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human EIF2S1 aa 50-150 conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human eIF2 alpha.
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Positive control
- Recombinant Human EIF2S1 protein (ab123468) can be used as a positive control in WB. HeLa Whole Cell Lysate Jurkat Whole Cell Lysate A431 Whole Cell Lysate NIH 3T3 Whole Cell Lysate MEF1 Whole Cell Lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (6) |
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
Sequence similarities
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain. -
Post-translational
modificationsSubstrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. -
Cellular localization
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 32617 Drosophila melanogaster
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Entrez Gene: 54318 Rat
- Omim: 603907 Human
- SwissProt: P41374 Drosophila melanogaster
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
see all -
Alternative names
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
Images
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All lanes : Anti-EIF2S1 antibody (ab26197) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDaab26197 detects a band of ~ 36 kDa in HeLa, Jurkat and A431 whole cell lysates. This corresponds to the predicted molecular weight for eIF2 alpha according to Swiss prot. A number of other bands are detected at ~ 48, 23 and 15 kDa. We do not know the identity of these bands and we predict they represent non-specific binding.
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All lanes : Anti-EIF2S1 antibody (ab26197) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa -
ICC/IF image of ab26197 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab26197, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab26197 has been referenced in 37 publications.
- Bergquist H et al. Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I. Int J Mol Sci 23:N/A (2022). PubMed: 35328524
- Pitera AP et al. Cellular responses to halofuginone reveal a vulnerability of the GCN2 branch of the integrated stress response. EMBO J 41:e109985 (2022). PubMed: 35466425
- Popovic R et al. Combined Transcriptomic and Proteomic Analysis of Perk Toxicity Pathways. Int J Mol Sci 22:N/A (2021). PubMed: 33925631
- Peña OA et al. TLR7 ligation augments hematopoiesis in Rps14 (uS11) deficiency via paradoxical suppression of inflammatory signaling. Blood Adv 5:4112-4124 (2021). PubMed: 34432872
- Martinez-Miguel VE et al. Increased fidelity of protein synthesis extends lifespan. Cell Metab 33:2288-2300.e12 (2021). PubMed: 34525330