Key features and details
- Rabbit polyclonal to EIF2S1 (phospho S51)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-EIF2S1 (phospho S51) antibody
See all EIF2S1 primary antibodies
DescriptionRabbit polyclonal to EIF2S1 (phospho S51)
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- HeLa cells, K562 cells treated with IFN-alpha, Human breast carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purifed by affinity chromatography using epitope-specific phospho peptide. Non-phospho specific antibodies were removed using non-phospho peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab131505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa.Can be blocked with EIF2S1 (phospho S51) peptide (ab263896).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Sequence similaritiesBelongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.
modificationsSubstrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
Cellular localizationCytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
- Information by UniProt
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
All lanes : Anti-EIF2S1 (phospho S51) antibody (ab131505) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract treated with IFN alpha
Predicted band size: 36 kDa
Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131505 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelled with ab131505 at 1/50 dilution (left) or ab131505 preincubated with blocking peptide (right).
ab131505 has been referenced in 1 publication.
- Aulas A et al. Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability. Cell Death Dis 9:1129 (2018). PubMed: 30425239