Recombinant
RabMAb

Recombinant Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Rabbit recombinant monoclonal EIF2S1 (phospho S51) antibody [E90]. Validated in WB, IHC, Flow Cyt, Dot, ICC/IF and tested in Mouse, Rat, Human, Drosophila melanogaster, Plants and more.

Publishing research using ab32157? Please let us know so that we can cite the reference in this datasheet.

ab32157 has been referenced in 105 publications.

  • Yadav AK  et al. AZD1208, a Pan-Pim Kinase Inhibitor, Has Anti-Growth Effect on 93T449 Human Liposarcoma Cells via Control of the Expression and Phosphorylation of Pim-3, mTOR, 4EBP-1, S6, STAT-3 and AMPK. Int J Mol Sci 20:N/A (2019). PubMed: 30654529
  • Cagnetta R  et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434
  • Westergard T  et al. Repeat-associated non-AUG translation in C9orf72-ALS/FTD is driven by neuronal excitation and stress. EMBO Mol Med 11:N/A (2019). PubMed: 30617154
  • Rabouw HH  et al. Small molecule ISRIB suppresses the integrated stress response within a defined window of activation. Proc Natl Acad Sci U S A 116:2097-2102 (2019). PubMed: 30674674
  • Fusade-Boyer M  et al. Evaluation of the Antiviral Activity of Sephin1 Treatment and Its Consequences on eIF2a Phosphorylation in Response to Viral Infections. Front Immunol 10:134 (2019). PubMed: 30809223
  • Patel S  et al. GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans. Cell Metab 29:707-718.e8 (2019). PubMed: 30639358
  • Cormerais Y  et al. Inhibition of the amino-acid transporter LAT1 demonstrates anti-neoplastic activity in medulloblastoma. J Cell Mol Med 23:2711-2718 (2019). PubMed: 30784173
  • Liu P  et al. Crizotinib-induced immunogenic cell death in non-small cell lung cancer. Nat Commun 10:1486 (2019). PubMed: 30940805
  • Xie ZY  et al. Acid-Sensing Ion Channel 1a Regulates Fate of Rat Nucleus Pulposus Cells in Acid Stimulus Through Endoplasmic Reticulum Stress. Biores Open Access 7:2-9 (2018). PubMed: 29445584
  • Liu S  et al. Gastrodin alleviates glucocorticoid induced osteoporosis in rats via activating the Nrf2 signaling pathways. Oncotarget 9:11528-11540 (2018). PubMed: 29545917
  • Chen L  et al. Nuclear factor-kappa B-dependent X-box binding protein 1 signalling promotes the proliferation of nucleus pulposus cells under tumour necrosis factor alpha stimulation. Cell Prolif N/A:e12542 (2018). PubMed: 30430692
  • Chen L  et al. Protein kinase RNA-like ER kinase/eukaryotic translation initiation factor 2a pathway attenuates tumor necrosis factor alpha-induced apoptosis in nucleus pulposus cells by activating autophagy. J Cell Physiol N/A:N/A (2018). PubMed: 30515797
  • Park S  et al. Modulation of Protein Synthesis by eIF2a Phosphorylation Protects Cell from Heat Stress-Mediated Apoptosis. Cells 7:N/A (2018). PubMed: 30544621
  • Yang F  et al. ER-stress regulates macrophage polarization through pancreatic EIF-2alpha kinase. Cell Immunol N/A:N/A (2018). PubMed: 30594305
  • Xue M  et al. The PERK Arm of the Unfolded Protein Response Negatively Regulates Transmissible Gastroenteritis Virus Replication by Suppressing Protein Translation and Promoting Type I Interferon Production. J Virol 92:N/A (2018). PubMed: 29769338
  • Tuorto F  et al. Queuosine-modified tRNAs confer nutritional control of protein translation. EMBO J 37:N/A (2018). PubMed: 30093495
  • Liu H  et al. Oxidative Stress Mediates Microcystin-LR-Induced Endoplasmic Reticulum Stress and Autophagy in KK-1 Cells and C57BL/6 Mice Ovaries. Front Physiol 9:1058 (2018). PubMed: 30131715
  • Sadleir KR  et al. ER stress is not elevated in the 5XFAD mouse model of Alzheimer's disease. J Biol Chem 293:18434-18443 (2018). PubMed: 30315100
  • Collier AE  et al. Translational control of a human CDKN1A mRNA splice variant regulates the fate of UVB-irradiated human keratinocytes. Mol Biol Cell 29:29-41 (2018). PubMed: 29118075
  • Meng C  et al. PERK Pathway Activation Promotes Intracerebral Hemorrhage Induced Secondary Brain Injury by Inducing Neuronal Apoptosis Both in Vivo and in Vitro. Front Neurosci 12:111 (2018). PubMed: 29541018
  • Thongon N  et al. Cancer cell metabolic plasticity allows resistance to NAMPT inhibition but invariably induces dependence on LDHA. Cancer Metab 6:1 (2018). PubMed: 29541451
  • Edenharter O  et al. Mitofusin-Dependent ER Stress Triggers Glial Dysfunction and Nervous System Degeneration in a Drosophila Model of Friedreich's Ataxia. Front Mol Neurosci 11:38 (2018). PubMed: 29563863
  • Ebersbach-Silva P  et al. Palmitate-induced Slc2a4/GLUT4 downregulation in L6 muscle cells: evidence of inflammatory and endoplasmic reticulum stress involvement. Lipids Health Dis 17:64 (2018). PubMed: 29609616
  • Sun P  et al. Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1. Autophagy 14:336-346 (2018). PubMed: 29166823
  • Ren W  et al. Arginine inhibits the malignant transformation induced by interferon-gamma through the NF-?B-GCN2/eIF2a signaling pathway in mammary epithelial cells in vitro and in vivo. Exp Cell Res 368:236-247 (2018). Cow . PubMed: 29746817
  • He Y  et al. Palmitic acid, but not high-glucose, induced myocardial apoptosis is alleviated by N-acetylcysteine due to attenuated mitochondrial-derived ROS accumulation-induced endoplasmic reticulum stress. Cell Death Dis 9:568 (2018). PubMed: 29752433
  • Moon SL & Parker R EIF2B2 mutations in vanishing white matter disease hypersuppress translation and delay recovery during the integrated stress response. RNA 24:841-852 (2018). PubMed: 29632131
  • Yuan X  et al. Allergy immunotherapy restores airway epithelial barrier dysfunction through suppressing IL-25 -induced endoplasmic reticulum stress in asthma. Sci Rep 8:7950 (2018). PubMed: 29784924
  • Shelkovnikova TA  et al. Protective paraspeckle hyper-assembly downstream of TDP-43 loss of function in amyotrophic lateral sclerosis. Mol Neurodegener 13:30 (2018). PubMed: 29859124
  • Sauvat A  et al. Trans-Fats Inhibit Autophagy Induced by Saturated Fatty Acids. EBioMedicine 30:261-272 (2018). ICC/IF . PubMed: 29606629
  • Li C  et al. The three branches of the unfolded protein response exhibit differential significance in breast cancer growth and stemness. Exp Cell Res 367:170-185 (2018). IHC-P . PubMed: 29601799
  • Chi L  et al. Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model. Med Sci Monit 24:3946-3957 (2018). PubMed: 29888735
  • Li H  et al. Apoptosis Induction by Iron Radiation via Inhibition of Autophagy in Trp53+/- Mouse Testes: Is Chronic Restraint-Induced Stress a Modifying Factor? Int J Biol Sci 14:1109-1121 (2018). PubMed: 29989073
  • Yang F  et al. IRE1a aggravates ischemia reperfusion injury of fatty liver by regulating phenotypic transformation of kupffer cells. Free Radic Biol Med 124:395-407 (2018). PubMed: 29969718
  • Topf U  et al. Quantitative proteomics identifies redox switches for global translation modulation by mitochondrially produced reactive oxygen species. Nat Commun 9:324 (2018). PubMed: 29358734
  • Cormerais Y  et al. The glutamine transporter ASCT2 (SLC1A5) promotes tumor growth independently of the amino acid transporter LAT1 (SLC7A5). J Biol Chem 293:2877-2887 (2018). PubMed: 29326164
  • Takahashi H  et al. Identification of functional features of synthetic SINEUPs, antisense lncRNAs that specifically enhance protein translation. PLoS One 13:e0183229 (2018). PubMed: 29414979
  • Pareek G  et al. Loss of the Drosophila m-AAA mitochondrial protease paraplegin results in mitochondrial dysfunction, shortened lifespan, and neuronal and muscular degeneration. Cell Death Dis 9:304 (2018). PubMed: 29467464
  • Crespillo-Casado A  et al. PPP1R15A-mediated dephosphorylation of eIF2a is unaffected by Sephin1 or Guanabenz. Elife 6:N/A (2017). PubMed: 28447936
  • Sun D  et al. Administration of Tauroursodeoxycholic Acid Attenuates Early Brain Injury via Akt Pathway Activation. Front Cell Neurosci 11:193 (2017). PubMed: 28729823
  • Dimasi P  et al. Modulation of p-eIF2a cellular levels and stress granule assembly/disassembly by trehalose. Sci Rep 7:44088 (2017). PubMed: 28276506
  • Woo M  et al. Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis. Nutr Res Pract 11:445-451 (2017). PubMed: 29209454
  • Thiessen SE  et al. The Role of Autophagy in Critical Illness-induced Liver Damage. Sci Rep 7:14150 (2017). PubMed: 29074879
  • Dong Y  et al. Sulfur availability regulates plant growth via glucose-TOR signaling. Nat Commun 8:1174 (2017). PubMed: 29079776
  • Lev A  et al. Anti-pancreatic cancer activity of ONC212 involves the unfolded protein response (UPR) and is reduced by IGF1-R and GRP78/BIP. Oncotarget 8:81776-81793 (2017). PubMed: 29137221
  • Bailey KA  et al. Atherosusceptible Shear Stress Activates Endoplasmic Reticulum Stress to Promote Endothelial Inflammation. Sci Rep 7:8196 (2017). PubMed: 28811527
  • Clayton BL  et al. The integrated stress response in hypoxia-induced diffuse white matter injury. J Neurosci N/A:N/A (2017). PubMed: 28720571
  • Jennings MD  et al. Fail-safe control of translation initiation by dissociation of eIF2a phosphorylated ternary complexes. Elife 6:N/A (2017). WB . PubMed: 28315520
  • Perego J  et al. Guanabenz Prevents d-Galactosamine/Lipopolysaccharide-Induced Liver Damage and Mortality. Front Immunol 8:679 (2017). WB ; Mouse . PubMed: 28659918
  • Mitchell JK  et al. Hepatitis C Virus Indirectly Disrupts DNA Damage-Induced p53 Responses by Activating Protein Kinase R. MBio 8:N/A (2017). PubMed: 28442604
  • Shelkovnikova TA  et al. Chronically stressed or stress-preconditioned neurons fail to maintain stress granule assembly. Cell Death Dis 8:e2788 (2017). IHC-P ; Mouse . PubMed: 28492545
  • Yan L  et al. Icariside II Effectively Reduces Spatial Learning and Memory Impairments in Alzheimer's Disease Model Mice Targeting Beta-Amyloid Production. Front Pharmacol 8:106 (2017). PubMed: 28337142
  • Li S  et al. Oxidative stress drives CD8+ T-cell skin trafficking in patients with vitiligo through CXCL16 upregulation by activating the unfolded protein response in keratinocytes. J Allergy Clin Immunol 140:177-189.e9 (2017). PubMed: 27826097
  • Humphries MP  et al. A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer. Clin Cancer Res 23:2575-2583 (2017). PubMed: 27986751
  • Pagliassotti MJ  et al. Trehalose supplementation reduces hepatic endoplasmic reticulum stress and inflammatory signaling in old mice. J Nutr Biochem 45:15-23 (2017). PubMed: 28431320
  • Yadav V  et al. Hydrogen sulfide modulates eukaryotic translation initiation factor 2a (eIF2a) phosphorylation status in the integrated stress-response pathway. J Biol Chem 292:13143-13153 (2017). PubMed: 28637872
  • Celardo I  et al. Mitofusin-mediated ER stress triggers neurodegeneration in pink1/parkin models of Parkinson's disease. Cell Death Dis 7:e2271 (2016). PubMed: 27336715
  • Backlund M  et al. Endoplasmic reticulum stress increases AT1R mRNA expression via TIA-1-dependent mechanism. Nucleic Acids Res 44:3095-104 (2016). WB ; Human . PubMed: 26681690
  • Xia X  et al. Arginine Supplementation Recovered the IFN-?-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2a Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells. Mol Cells 39:410-7 (2016). WB . PubMed: 27025389
  • Kline CL  et al. ONC201 kills solid tumor cells by triggering an integrated stress response dependent on ATF4 activation by specific eIF2a kinases. Sci Signal 9:ra18 (2016). WB . PubMed: 26884600
  • Averous J  et al. GCN2 contributes to mTORC1 inhibition by leucine deprivation through an ATF4 independent mechanism. Sci Rep 6:27698 (2016). PubMed: 27297692
  • Lee YH  et al. Association between betatrophin/ANGPTL8 and non-alcoholic fatty liver disease: animal and human studies. Sci Rep 6:24013 (2016). WB . PubMed: 27045862
  • Qi Y  et al. Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration. Cell Death Differ 23:291-302 (2016). PubMed: 26160069
  • Park IA  et al. Endoplasmic reticulum stress induces secretion of high-mobility group proteins and is associated with tumor-infiltrating lymphocytes in triple-negative breast cancer. Oncotarget 7:59957-59964 (2016). IHC . PubMed: 27494867
  • Szaflarski W  et al. Vinca alkaloid drugs promote stress-induced translational repression and stress granule formation. Oncotarget 7:30307-22 (2016). PubMed: 27083003
  • Fusakio ME  et al. Transcription factor ATF4 directs basal and stress-induced gene expression in the unfolded protein response and cholesterol metabolism in the liver. Mol Biol Cell 27:1536-51 (2016). WB . PubMed: 26960794
  • Cormerais Y  et al. Genetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth. Cancer Res 76:4481-92 (2016). WB ; Human . PubMed: 27302165
  • Seiferling D  et al. Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt. EMBO Rep 17:953-64 (2016). WB . PubMed: 27154400
  • Elder MJ  et al. TSLP production by dendritic cells is modulated by IL-1ß and components of the endoplasmic reticulum stress response. Eur J Immunol 46:455-63 (2016). PubMed: 26573878
  • Young SK  et al. Ribosome Elongation Stall Directs Gene-specific Translation in the Integrated Stress Response. J Biol Chem 291:6546-58 (2016). PubMed: 26817837
  • Young SK  et al. Translation Regulation of the Glutamyl-prolyl-tRNA Synthetase Gene EPRS through Bypass of Upstream Open Reading Frames with Noncanonical Initiation Codons. J Biol Chem 291:10824-35 (2016). PubMed: 27002157
  • Young SK  et al. Nuclear Matrix Protein 4 Is a Novel Regulator of Ribosome Biogenesis and Controls the Unfolded Protein Response via Repression of Gadd34 Expression. J Biol Chem 291:13780-8 (2016). PubMed: 27129771
  • Ladoire S  et al. Biomarkers of immunogenic stress in metastases from melanoma patients: Correlations with the immune infiltrate. Oncoimmunology 5:e1160193 (2016). PubMed: 27471635
  • Golovko A  et al. The eIF2A knockout mouse. Cell Cycle 15:3115-3120 (2016). PubMed: 27686860
  • Bastola P  et al. VCP inhibitors induce endoplasmic reticulum stress, cause cell cycle arrest, trigger caspase-mediated cell death and synergistically kill ovarian cancer cells in combination with Salubrinal. Mol Oncol 10:1559-1574 (2016). PubMed: 27729194
  • Semeraro M  et al. The ratio of CD8+/FOXP3 T lymphocytes infiltrating breast tissues predicts the relapse of ductal carcinoma in situ. Oncoimmunology 5:e1218106 (2016). PubMed: 27853639
  • Zucal C  et al. EIF2A-dependent translational arrest protects leukemia cells from the energetic stress induced by NAMPT inhibition. BMC Cancer 15:855 (2015). WB ; Human . PubMed: 26542945
  • Vaughn LS  et al. Altered activation of protein kinase PKR and enhanced apoptosis in dystonia cells carrying a mutation in PKR activator protein PACT. J Biol Chem 290:22543-57 (2015). PubMed: 26231208
  • Tseng YY  et al. Functional analysis of the short isoform of orf virus protein OV20.0. J Virol 89:4966-79 (2015). PubMed: 25694596
  • Portovedo M  et al. Saturated fatty acids modulate autophagy's proteins in the hypothalamus. PLoS One 10:e0119850 (2015). PubMed: 25786112
  • Way SW  et al. Pharmaceutical integrated stress response enhancement protects oligodendrocytes and provides a potential multiple sclerosis therapeutic. Nat Commun 6:6532 (2015). WB . PubMed: 25766071
  • Ghosh S & Geahlen RL Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer's Disease. EBioMedicine 2:1785-98 (2015). PubMed: 26870803
  • Young SK  et al. Ribosome Reinitiation Directs Gene-specific Translation and Regulates the Integrated Stress Response. J Biol Chem 290:28257-71 (2015). PubMed: 26446796
  • Wengrod J  et al. Phosphorylation of eIF2a triggered by mTORC1 inhibition and PP6C activation is required for autophagy and is aberrant in PP6C-mutated melanoma. Sci Signal 8:ra27 (2015). WB ; Human . PubMed: 25759478
  • Carrara M  et al. Crystal structures reveal transient PERK luminal domain tetramerization in endoplasmic reticulum stress signaling. EMBO J 34:1589-600 (2015). Mouse . PubMed: 25925385
  • Hsu WL  et al. The untranslated regions of classic swine fever virus RNA trigger apoptosis. PLoS One 9:e88863 (2014). WB . PubMed: 24533157
  • Sadleir KR  et al. Genetic inhibition of phosphorylation of the translation initiation factor eIF2a does not block Aß-dependent elevation of BACE1 and APP levels or reduce amyloid pathology in a mouse model of Alzheimer's disease. PLoS One 9:e101643 (2014). WB ; Mouse . PubMed: 24992504
  • Kosakowska-Cholody T  et al. HKH40A downregulates GRP78/BiP expression in cancer cells. Cell Death Dis 5:e1240 (2014). Human . PubMed: 24853418
  • Nallagatla SR  et al. Native tertiary structure and nucleoside modifications suppress tRNA's intrinsic ability to activate the innate immune sensor PKR. PLoS One 8:e57905 (2013). WB . PubMed: 23483938
  • Banduseela VC  et al. Impaired autophagy, chaperone expression, and protein synthesis in response to critical illness interventions in porcine skeletal muscle. Physiol Genomics 45:477-86 (2013). PubMed: 23572537
  • B'chir W  et al. The eIF2a/ATF4 pathway is essential for stress-induced autophagy gene expression. Nucleic Acids Res 41:7683-99 (2013). WB ; Mouse . PubMed: 23804767
  • Yang PM  et al. Zebularine inhibits tumorigenesis and stemness of colorectal cancer via p53-dependent endoplasmic reticulum stress. Sci Rep 3:3219 (2013). WB ; Human . PubMed: 24225777
  • Coope A  et al. Chaperone insufficiency links TLR4 protein signaling to endoplasmic reticulum stress. J Biol Chem 287:15580-9 (2012). WB ; Mouse . PubMed: 22427667
  • Tardito S  et al. L-Asparaginase and inhibitors of glutamine synthetase disclose glutamine addiction of ß-catenin-mutated human hepatocellular carcinoma cells. Curr Cancer Drug Targets 11:929-43 (2011). WB ; Human . PubMed: 21834755
  • Vanderperre B  et al. An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein. FASEB J 25:2373-86 (2011). PubMed: 21478263
  • Zhang F  et al. The African swine fever virus DP71L protein recruits the protein phosphatase 1 catalytic subunit to dephosphorylate eIF2alpha and inhibits CHOP induction but is dispensable for these activities during virus infection. J Virol 84:10681-9 (2010). PubMed: 20702639
  • Xu YX  et al. Inhibition of Golgi apparatus glycosylation causes endoplasmic reticulum stress and decreased protein synthesis. J Biol Chem 285:24600-8 (2010). PubMed: 20529871
  • Cantero-Recasens G  et al. The asthma-associated ORMDL3 gene product regulates endoplasmic reticulum-mediated calcium signaling and cellular stress. Hum Mol Genet 19:111-21 (2010). WB ; Human . PubMed: 19819884
  • Gorbatyuk MS  et al. Restoration of visual function in P23H rhodopsin transgenic rats by gene delivery of BiP/Grp78. Proc Natl Acad Sci U S A 107:5961-6 (2010). WB ; Rat . PubMed: 20231467
  • Konsavage WM  et al. Hyperoxia Inhibits Protein Synthesis and Increases eIF2{alpha} Phosphorylation in the Newborn Rat Lung. Am J Physiol Lung Cell Mol Physiol : (2010). IHC-Fr ; Rat . PubMed: 20139179
  • Duffy C  et al. VP22 of herpes simplex virus 1 promotes protein synthesis at late times in infection and accumulation of a subset of viral mRNAs at early times in infection. J Virol 83:1009-17 (2009). WB ; African green monkey . PubMed: 18987147
  • Farny NG  et al. Metazoan stress granule assembly is mediated by P-eIF2alpha-dependent and -independent mechanisms. RNA 15:1814-21 (2009). WB ; Drosophila melanogaster . PubMed: 19661161
  • Parlati F  et al. Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasome. Blood 114:3439-47 (2009). WB ; Human . PubMed: 19671918
  • Kren BT  et al. Polysome trafficking of transcripts and microRNAs in regenerating liver after partial hepatectomy. Am J Physiol Gastrointest Liver Physiol 297:G1181-92 (2009). PubMed: 19779017
  • Wu D  et al. 2-Aminopurine inhibits lipid accumulation induced by apolipoprotein E-deficient lipoprotein in macrophages: potential role of eukaryotic initiation factor-2alpha phosphorylation in foam cell formation. J Pharmacol Exp Ther 326:395-405 (2008). PubMed: 18463320

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