Name: Anti-EIF2S1 (phospho S51) antibody [E90]
Brand: RabMAb
SKU: ab32157
Rating: 4 (24 reviews)

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Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
Suitable for: WB, IHC-P, Dot blot
Reacts with: Rat, Human, Neurospora crassa

Product name

Anti-EIF2S1 (phospho S51) antibody [E90]
See all EIF2S1 primary antibodies
Description

Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
Host species

Rabbit
Specificity

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51.

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

corresponding to Human EIF2S1 (phospho S51).
Database link: P05198
(Peptide available as ab199382)
Positive control
- IHC-P: Human liver carcinoma, breast carcinoma, cervical carcinoma, colon adenocarcinoma and hepatocellular carcinoma tissues. WB: HeLa cells treated with Clyculin A and phosphatase whole cell lysate; PC-12 cell lysate. Dot Blot: Antigen peptide.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

E90
Isotype

IgG
Research areas

Alternative Versions
- Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (ab214434)
Compatible Secondaries
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
IHC-P	Human
WB	Human
All applications	Mouse Cow Pig Drosophila melanogaster Monkey Plants African green monkey

Application	Abreviews	Notes
WB	(19)	1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P	(1)	1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot		1/500.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot 1/500.

Application notes

Is unsuitable for Flow Cyt,ICC or IP.

Function

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Sequence similarities

Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.
Post-translational
modifications

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
Cellular localization

Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
Target information above from: UniProt accession P05198 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 327694 Cow
- Entrez Gene: 32617 Drosophila melanogaster
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Entrez Gene: 54318 Rat
- Omim: 603907 Human
- SwissProt: P41374 Drosophila melanogaster
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
- SwissProt: P68101 Rat
- Unigene: 3157 Drosophila melanogaster
- Unigene: 151777 Human
- Unigene: 196220 Mouse
- Unigene: 1488 Rat
see all
Alternative names
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
- EIF 2alpha antibody
- eIF-2-alpha antibody
- eIF-2A antibody
- EIF-2alpha antibody
- EIF2 alpha antibody
- EIF2 antibody
- EIF2A antibody
- EIF2S1 antibody
- Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
- Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
- Eukaryotic translation initiation factor 2 subunit 1 antibody
- Eukaryotic translation initiation factor 2 subunit alpha antibody
- IF2A_HUMAN antibody
see all

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/20000 dilution

Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa treated with Calyculin A whole cell lysates
Lane 3 : HeLa treated with Calyculin A and phosphatase whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking buffer and concentration: 2% BSA/TBST.

Diluting buffer and concentration: 2% BSA /TBST.

Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.
Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate

Predicted band size: 36 kDa
Observed band size: 36 kDa

Secondary antibody - anti-rabbit HRP (ab6721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Breast carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Cervical carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Colonic adenocarcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

ab32157 showing positive staining in Hepatocellular carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Publishing research using ab32157? Please let us know so that we can cite the reference in this datasheet.

ab32157 has been referenced in 135 publications.

Klann K et al. Functional Translatome Proteomics Reveal Converging and Dose-Dependent Regulation by mTORC1 and eIF2a. Mol Cell 77:913-925.e4 (2020). PubMed: 31812349
Kapoor A et al. Endorepellin evokes an angiostatic stress signaling cascade in endothelial cells. J Biol Chem 295:6344-6356 (2020). PubMed: 32205445
Yoon J & Rhee K Whole-body heat exposure causes developmental stage-specific apoptosis of male germ cells. Mol Reprod Dev 87:680-691 (2020). PubMed: 32506506
Tian M et al. Dietary Branched-Chain Amino Acids Regulate Food Intake Partly through Intestinal and Hypothalamic Amino Acid Receptors in Piglets. J Agric Food Chem 67:6809-6818 (2019). PubMed: 31134808
Fuentes-Villalobos F et al. DISC1 promotes translation maintenance during sodium arsenite-induced oxidative stress. Biochim Biophys Acta Gene Regul Mech 1862:657-669 (2019). PubMed: 31075539

View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Human Primary Fibroblasts)

Gel Running Conditions

Reduced Denaturing (12% TGX)

Loading amount

10 µg

Treatment

different treatments

Specification

Human Primary Fibroblasts

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 21 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa)

Gel Running Conditions

Reduced Denaturing (8 %)

Loading amount

20 µg

Treatment

Thapsigargin (0.5 uM)

Specification

HeLa

Blocking step

Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Jin Ik Kim

Verified customer

Submitted Apr 20 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (MDA231 cells)

Gel Running Conditions

Reduced Denaturing (4-20% Tris Glycin gel)

Loading amount

800000 cells

Specification

MDA231 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 19 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (lymphocytes)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris)

Loading amount

15 µg

Treatment

4ug/mL Tunicamycin or DMSO for 2 or 8 hours

Specification

lymphocytes

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 12 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (HepG2 cell line)

Loading amount

30 µg

Specification

HepG2 cell line

Treatment

Asparaginase 1U/ml for 9h

Gel Running Conditions

Reduced Denaturing (gel 10%)

Blocking step

blocking solution (Roche as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Martina Chiu

Verified customer

Submitted Jan 17 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa cell line)

Loading amount

20 µg

Specification

HeLa cell line

Gel Running Conditions

Reduced Denaturing

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C

Dr. Mirjam Eckert

Verified customer

Submitted Apr 02 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Overview

Product name

Description

Host species

Specificity

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (135)

Customer reviews and Q&As

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Post-translational
modifications