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  1. Link

    eif2s1-phospho-s51-antibody-e90-ab32157.pdf

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RecombinantRabMAb

Recombinant Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

  • Datasheet
  • SDS
Reviews (25)Q&A (2)References (189)

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Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human, Neurospora crassa

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-EIF2S1 (phospho S51) antibody [E90]
    See all EIF2S1 primary antibodies
  • Description

    Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
  • Host species

    Rabbit
  • Specificity

    Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51.

  • Tested applications

    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Neurospora crassa
    Predicted to work with: Cow, Pig, Drosophila melanogaster, Monkey, Plants, African green monkey
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver carcinoma, breast carcinoma, cervical carcinoma, colon adenocarcinoma and hepatocellular carcinoma tissues. WB: HeLa cells treated with Clyculin A and phosphatase whole cell lysate; PC-12 cell lysate. Dot Blot: Antigen peptide.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E90
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Associated products

  • Alternative Versions

    • Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (ab214434)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (19)
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P (2)
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot
1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot
1/500.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities

    Belongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • Post-translational
    modifications

    Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • Cellular localization

    Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Target information above from: UniProt accession P05198 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 327694 Cow
    • Entrez Gene: 32617 Drosophila melanogaster
    • Entrez Gene: 1965 Human
    • Entrez Gene: 13665 Mouse
    • Entrez Gene: 54318 Rat
    • Omim: 603907 Human
    • SwissProt: P41374 Drosophila melanogaster
    • SwissProt: P05198 Human
    • SwissProt: Q6ZWX6 Mouse
    • SwissProt: P68101 Rat
    • Unigene: 3157 Drosophila melanogaster
    • Unigene: 151777 Human
    • Unigene: 196220 Mouse
    • Unigene: 1488 Rat
    see all
  • Alternative names

    • EIF 2 alpha antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • EIF 2alpha antibody
    • eIF-2-alpha antibody
    • eIF-2A antibody
    • EIF-2alpha antibody
    • EIF2 alpha antibody
    • EIF2 antibody
    • EIF2A antibody
    • EIF2S1 antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    • IF2A_HUMAN antibody
    see all

Images

  • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) Whole cell lysates
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with alkaline phosphatase.
    Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with lambda phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 180 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    Immunohistochemical analysis of paraffin-embedded human lung cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

    Positive staining on human lung cancer without alkaline phosphatase treatment (image A). No staining on human lung cancer with alkaline phosphatase treatment (image B)
    The section was incubated with ab32157 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

     

  • Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    Dot blot analysis of EIF2S1 (pS51) phospho peptide (Lane 1), EIF2S1 non-phospho peptide (Lane 2) with ab32157 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/20000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa treated with Calyculin A whole cell lysates
    Lane 3 : HeLa treated with Calyculin A and phosphatase whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking buffer and concentration: 2% BSA/TBST.

    Diluting buffer and concentration: 2% BSA /TBST.

    Exposure time: 10 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

    Positive staining on mouse pancreatic cancer without alkaline phosphatase treatment (image A). No staining on mouse pancreatic cancer with alkaline phosphatase treatment (image B)
    The section was incubated with ab32157 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.
  • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    ab32157 showing positive staining in Breast carcinoma tissue at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    ab32157 showing positive staining in Cervical carcinoma tissue at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    ab32157 showing positive staining in Colonic adenocarcinoma tissue at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    ab32157 showing positive staining in Hepatocellular carcinoma tissue at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
    Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (189)

Publishing research using ab32157? Please let us know so that we can cite the reference in this datasheet.

ab32157 has been referenced in 189 publications.

  • Popovic R  et al. Combined Transcriptomic and Proteomic Analysis of Perk Toxicity Pathways. Int J Mol Sci 22:N/A (2021). PubMed: 33925631
  • Huang Y  et al. Elucidation of the mechanism of NEFA-induced PERK-eIF2a signaling pathway regulation of lipid metabolism in bovine hepatocytes. J Steroid Biochem Mol Biol 211:105893 (2021). PubMed: 33819629
  • Arioka Y  et al. Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons. EBioMedicine 63:103138 (2021). PubMed: 33341442
  • Tian AL  et al. Lysosomotropic agents including azithromycin, chloroquine and hydroxychloroquine activate the integrated stress response. Cell Death Dis 12:6 (2021). PubMed: 33414432
  • Zyryanova AF  et al. ISRIB Blunts the Integrated Stress Response by Allosterically Antagonising the Inhibitory Effect of Phosphorylated eIF2 on eIF2B. Mol Cell 81:88-103.e6 (2021). PubMed: 33220178
View all Publications for this product

Customer reviews and Q&As

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1-10 of 27 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mice Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
mice Pancreas
Blocking step
Bloxall as blocking agent for 20 minute(s) · Concentration: 100%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 06 2022

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart, Liver)
Gel Running Conditions
Reduced Denaturing (12% TGX)
Loading amount
20 µg
Specification
Heart, Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 22 2021

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Unfertilised Xenopus oocytes, stage V-VI)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
0.5 cells
Specification
Unfertilised Xenopus oocytes, stage V-VI
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Rowena Martin

Verified customer

Submitted Nov 17 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Below Average
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Primary Fibroblasts)
Permeabilization
Yes - 0.1% Triton 1h
Specification
Human Primary Fibroblasts
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 21 2020

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Primary Fibroblasts)
Gel Running Conditions
Reduced Denaturing (12% TGX)
Loading amount
10 µg
Treatment
different treatments
Specification
Human Primary Fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 21 2020

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (tedpole's head)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
8 µg
Treatment
10uM octylphenol for 96hrs
Specification
tedpole's head
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Kyung noh Yoon

Verified customer

Submitted May 09 2019

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (8 %)
Loading amount
20 µg
Treatment
Thapsigargin (0.5 uM)
Specification
HeLa
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Jin Ik Kim

Verified customer

Submitted Apr 20 2019

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pichia pastoris Cell lysate - whole cell (Pichia Pastoris GS115 (his-))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
4.4 µg
Specification
Pichia Pastoris GS115 (his-)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 07 2018

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (RK13 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
200000 cells
Specification
RK13 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 25 2017

Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain Slices)
Gel Running Conditions
Reduced Denaturing (12% acrilamide)
Loading amount
20 µg
Treatment
2 ´g/ml for 4 hours
Specification
Brain Slices
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Berta Anuncibay

Verified customer

Submitted May 04 2017

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