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    eif2s1-phospho-s51-antibody-e90-ab32157.pdf

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RecombinantRabMAb

Recombinant Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

  • Datasheet
  • SDS
Reviews (24)Q&A (2)References (135)

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Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
  • Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Rat, Human, Neurospora crassa

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Overview

  • Product name

    Anti-EIF2S1 (phospho S51) antibody [E90]
    See all EIF2S1 primary antibodies
  • Description

    Rabbit monoclonal [E90] to EIF2S1 (phospho S51)
  • Host species

    Rabbit
  • Specificity

    Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human EIF2S1 (phospho S51).
    Database link: P05198
    (Peptide available as ab199382)

  • Positive control

    • IHC-P: Human liver carcinoma, breast carcinoma, cervical carcinoma, colon adenocarcinoma and hepatocellular carcinoma tissues. WB: HeLa cells treated with Clyculin A and phosphatase whole cell lysate; PC-12 cell lysate. Dot Blot: Antigen peptide.
  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E90
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Associated products

  • Alternative Versions

    • Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (ab214434)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Cow
Pig
Drosophila melanogaster
Monkey
Plants
African green monkey
Application Abreviews Notes
WB (19)
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P (1)
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot
1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot blot
1/500.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
    • Sequence similarities

      Belongs to the eIF-2-alpha family.
      Contains 1 S1 motif domain.
    • Post-translational
      modifications

      Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
    • Cellular localization

      Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
    • Target information above from: UniProt accession P05198 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 327694 Cow
      • Entrez Gene: 32617 Drosophila melanogaster
      • Entrez Gene: 1965 Human
      • Entrez Gene: 13665 Mouse
      • Entrez Gene: 54318 Rat
      • Omim: 603907 Human
      • SwissProt: P41374 Drosophila melanogaster
      • SwissProt: P05198 Human
      • SwissProt: Q6ZWX6 Mouse
      • SwissProt: P68101 Rat
      • Unigene: 3157 Drosophila melanogaster
      • Unigene: 151777 Human
      • Unigene: 196220 Mouse
      • Unigene: 1488 Rat
      see all
    • Alternative names

      • EIF 2 alpha antibody
      • EIF 2 antibody
      • EIF 2A antibody
      • EIF 2alpha antibody
      • eIF-2-alpha antibody
      • eIF-2A antibody
      • EIF-2alpha antibody
      • EIF2 alpha antibody
      • EIF2 antibody
      • EIF2A antibody
      • EIF2S1 antibody
      • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
      • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
      • Eukaryotic translation initiation factor 2 subunit 1 antibody
      • Eukaryotic translation initiation factor 2 subunit alpha antibody
      • IF2A_HUMAN antibody
      see all

    Images

    • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      All lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/20000 dilution

      Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates
      Lane 2 : HeLa treated with Calyculin A whole cell lysates
      Lane 3 : HeLa treated with Calyculin A and phosphatase whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa



      Blocking buffer and concentration: 2% BSA/TBST.

      Diluting buffer and concentration: 2% BSA /TBST.

      Exposure time: 10 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

      Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.
    • Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate

      Predicted band size: 36 kDa
      Observed band size: 36 kDa



      Secondary antibody - anti-rabbit HRP (ab6721)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

      ab32157 showing positive staining in Breast carcinoma tissue at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

      ab32157 showing positive staining in Cervical carcinoma tissue at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

      ab32157 showing positive staining in Colonic adenocarcinoma tissue at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

      ab32157 showing positive staining in Hepatocellular carcinoma tissue at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)
      Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (135)

    Publishing research using ab32157? Please let us know so that we can cite the reference in this datasheet.

    ab32157 has been referenced in 135 publications.

    • Klann K  et al. Functional Translatome Proteomics Reveal Converging and Dose-Dependent Regulation by mTORC1 and eIF2a. Mol Cell 77:913-925.e4 (2020). PubMed: 31812349
    • Kapoor A  et al. Endorepellin evokes an angiostatic stress signaling cascade in endothelial cells. J Biol Chem 295:6344-6356 (2020). PubMed: 32205445
    • Yoon J & Rhee K Whole-body heat exposure causes developmental stage-specific apoptosis of male germ cells. Mol Reprod Dev 87:680-691 (2020). PubMed: 32506506
    • Tian M  et al. Dietary Branched-Chain Amino Acids Regulate Food Intake Partly through Intestinal and Hypothalamic Amino Acid Receptors in Piglets. J Agric Food Chem 67:6809-6818 (2019). PubMed: 31134808
    • Fuentes-Villalobos F  et al. DISC1 promotes translation maintenance during sodium arsenite-induced oxidative stress. Biochim Biophys Acta Gene Regul Mech 1862:657-669 (2019). PubMed: 31075539
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Primary Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (12% TGX)
    Loading amount
    10 µg
    Treatment
    different treatments
    Specification
    Human Primary Fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 21 2020

    Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (8 %)
    Loading amount
    20 µg
    Treatment
    Thapsigargin (0.5 uM)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Jin Ik Kim

    Verified customer

    Submitted Apr 20 2019

    Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA231 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel)
    Loading amount
    800000 cells
    Specification
    MDA231 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 19 2016

    Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    15 µg
    Treatment
    4ug/mL Tunicamycin or DMSO for 2 or 8 hours
    Specification
    lymphocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2016

    Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2 cell line)
    Loading amount
    30 µg
    Specification
    HepG2 cell line
    Treatment
    Asparaginase 1U/ml for 9h
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Blocking step
    blocking solution (Roche as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Dr. Martina Chiu

    Verified customer

    Submitted Jan 17 2012

    Western blot abreview for Anti-EIF2S1 (phospho S51) antibody [E90]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cell line)
    Loading amount
    20 µg
    Specification
    HeLa cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Read More

    Dr. Mirjam Eckert

    Verified customer

    Submitted Apr 02 2009

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