Overview

  • Product name

    Anti-EIF2S2/EIF2B antibody
    See all EIF2S2/EIF2B primary antibodies
  • Description

    Rabbit polyclonal to EIF2S2/EIF2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human EIF2S2/EIF2B aa 119-331.
    Database link: P20042

  • Positive control

    • 293T, A431, H1299 and HeLa cell lysates; mouse liver lysate; rat PC12 and Rat2 cell lysates; HeLa cells; Human SG xenograft tissue.
  • General notes

     This product was previously labelled as EIF2S2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities

    Belongs to the eIF-2-beta/eIF-5 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686L18198 antibody
    • eIF 2 beta antibody
    • eIF-2-beta antibody
    • EIF2 antibody
    • EIF2B antibody
    • EIF2beta antibody
    • EIF2S2 antibody
    • Eukaryotic initiation factor 2 beta antibody
    • eukaryotic initiation factor 2-beta antibody
    • Eukaryotic translation initiation factor 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit 2 antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit beta antibody
    • eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa antibody
    • IF2B_HUMAN antibody
    • MGC8508 antibody
    • PPP1R67 antibody
    • protein phosphatase 1, regulatory subunit 67 antibody
    see all

Images

  • Paraffin-embedded mouse kidney tissue labeling EIF2S2/EIF2B using ab155649 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.

  • Anti-EIF2S2/EIF2B antibody (ab155649) at 1/1000 dilution + mouse liver lysate at 50 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • All lanes : Anti-EIF2S2/EIF2B antibody (ab155649) at 1/2000 dilution

    Lane 1 : PC12 whole cell lysate
    Lane 2 : Rat2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa



    10 % SDS-PAGE
  • All lanes : Anti-EIF2S2/EIF2B antibody (ab155649) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SG xenograft tissue, labeling EIF2S2/EIF2B using ab155649 at a 1/500 dilution.

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling EIF2S2/EIF2B using ab155649 at a 1/500 dilution. The image in the right panel was costained with Hoechst 33343.

References

ab155649 has not yet been referenced specifically in any publications.

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