Overview

  • Product name

    Anti-EIF2S2/EIF2B antibody
    See all EIF2S2/EIF2B primary antibodies
  • Description

    Rabbit polyclonal to EIF2S2/EIF2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human EIF2S2/EIF2B aa 283-333.
    Database link: NP_003899.2

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.
  • General notes

     This product was previously labelled as EIF2S2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab86105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities

    Belongs to the eIF-2-beta/eIF-5 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686L18198 antibody
    • eIF 2 beta antibody
    • eIF-2-beta antibody
    • EIF2 antibody
    • EIF2B antibody
    • EIF2beta antibody
    • EIF2S2 antibody
    • Eukaryotic initiation factor 2 beta antibody
    • eukaryotic initiation factor 2-beta antibody
    • Eukaryotic translation initiation factor 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit 2 antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit beta antibody
    • eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa antibody
    • IF2B_HUMAN antibody
    • MGC8508 antibody
    • PPP1R67 antibody
    • protein phosphatase 1, regulatory subunit 67 antibody
    see all

Images

  • All lanes : Anti-EIF2S2/EIF2B antibody (ab86105) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EIF2S2/EIF2B with ab86105 at 1/1000 (1µg/ml). Detection: DAB.

  • Detection of EIF2S2/EIF2B in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86105 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab86105 has not yet been referenced specifically in any publications.

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