Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15833(B)] to EIF2S2/EIF2B
- Suitable for: Flow Cyt, ICC/IF, IP, WB
- Reacts with: Human
Product nameAnti-EIF2S2/EIF2B antibody [EPR15833(B)]
See all EIF2S2/EIF2B primary antibodies
DescriptionRabbit monoclonal [EPR15833(B)] to EIF2S2/EIF2B
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human EIF2S2/EIF2B aa 100-200 (internal sequence). The exact sequence is proprietary.
Database link: P20042
- HepG2, HeLa, 293T and Jurkat cell lysates. HeLa and 293 cells.
This product was previously labelled as EIF2S2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/40 - 1/60.|
|WB||1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 38 kDa).|
FunctioneIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Sequence similaritiesBelongs to the eIF-2-beta/eIF-5 family.
- Information by UniProt
- DKFZp686L18198 antibody
- eIF 2 beta antibody
- eIF-2-beta antibody
All lanes : Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling EIF2S2/EIF2B using ab184549 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution, counterstained with Dapi.
Western blot analysis of EIF2S2/EIF2B in immunoprecipitation pellets from Jurkat lysate. ab184549 used at a 1/50 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution. Right lane shows PBS negative control.
Flow cytometric analysis of 2% paraformaldehyde fixed 293 cells labeling EIF2S2/EIF2B using ab184549 at a 1/160 dilution (red) or a Rabbit monoclonal IgG control (green).
Secondary antibody: Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution
ab184549 has not yet been referenced specifically in any publications.