Product nameAnti-eIF3A antibody [EPR5819(2)]
See all eIF3A primary antibodies
DescriptionRabbit monoclonal [EPR5819(2)] to eIF3A
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human eIF3A aa 1100-1200. The exact sequence is proprietary.
- BxPC3, 293T, Jurkat, C6, RAW264.7, PC12 and L6 lysates; Human brain tissue; BxPC3 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 8.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab128996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
Sequence similaritiesBelongs to the eIF-3 subunit A family.
Contains 1 PCI domain.
modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation.
- Information by UniProt
- centrosomin homolog antibody
- cytoplasmic protein p167 antibody
- eIF 3 theta antibody
All lanes : Anti-eIF3A antibody [EPR5819(2)] (ab128996) at 1/1000 dilution
Lane 1 : BxPC3 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 166 kDa
ab128996, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry.
ab128996, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Sonobe Y et al. Translation of dipeptide repeat proteins from the C9ORF72 expanded repeat is associated with cellular stress. Neurobiol Dis 116:155-165 (2018). Read more (PubMed: 29792928) »
- Luo CH et al. HE4 and eIF3a Expression Correlates with Surgical Outcome and Overall Survival in Ovarian Cancer Patients with Secondary Cytoreduction. J Cancer 9:2472-2479 (2018). Read more (PubMed: 30026845) »