Overview

  • Product name

    Anti-eIF3A antibody [EPR5819(2)]
    See all eIF3A primary antibodies
  • Description

    Rabbit monoclonal [EPR5819(2)] to eIF3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF3A aa 1100-1200. The exact sequence is proprietary.

  • Positive control

    • BxPC3, 293T, Jurkat, C6, RAW264.7, PC12 and L6 lysates; Human brain tissue; BxPC3 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
    • Sequence similarities

      Belongs to the eIF-3 subunit A family.
      Contains 1 PCI domain.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • centrosomin homolog antibody
      • cytoplasmic protein p167 antibody
      • eIF 3 theta antibody
      • eIF-3-theta antibody
      • EIF3 antibody
      • eIF3 p167 antibody
      • eIF3 p170 antibody
      • eIF3 p180 antibody
      • EIF3 p180 subunit antibody
      • eIF3 p185 antibody
      • eIF3 theta antibody
      • eIF3a antibody
      • EIF3A_HUMAN antibody
      • EIF3S10 antibody
      • eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) antibody
      • eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) antibody
      • eukaryotic translation initiation factor 3 subunit 10 170kDa antibody
      • Eukaryotic translation initiation factor 3 subunit 10 antibody
      • eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa antibody
      • Eukaryotic translation initiation factor 3 subunit A antibody
      • OTTHUMP00000020589 antibody
      • P167 antibody
      • p180 antibody
      • p185 antibody
      • TIF32 antibody
      see all

    Images

    • All lanes : Anti-eIF3A antibody [EPR5819(2)] (ab128996) at 1/1000 dilution

      Lane 1 : BxPC3 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : RAW264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : L6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 166 kDa

    • ab128996, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab128996, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Sonobe Y  et al. Translation of dipeptide repeat proteins from the C9ORF72 expanded repeat is associated with cellular stress. Neurobiol Dis 116:155-165 (2018). Read more (PubMed: 29792928) »
    • Luo CH  et al. HE4 and eIF3a Expression Correlates with Surgical Outcome and Overall Survival in Ovarian Cancer Patients with Secondary Cytoreduction. J Cancer 9:2472-2479 (2018). Read more (PubMed: 30026845) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab128996.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up