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    eif3a-antibody-n-terminal-ab155412.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-eIF3A antibody - N-terminal (ab155412)

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Western blot - Anti-eIF3A antibody - N-terminal (ab155412)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (ab155412)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody - N-terminal (ab155412)
  • Western blot - Anti-eIF3A antibody - N-terminal (ab155412)

Key features and details

  • Rabbit polyclonal to eIF3A - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-eIF3A antibody - N-terminal
    See all eIF3A primary antibodies
  • Description

    Rabbit polyclonal to eIF3A - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 121-347 of Human eIF3A (Uniprot: Q14152)

  • Positive control

    • 293T; A431; Jurkat or Raji cell lysates, Hepatoma tissue, A431 cells
  • General notes

    Keep as a concentrated solution

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 166 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 166 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
  • Sequence similarities

    Belongs to the eIF-3 subunit A family.
    Contains 1 PCI domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q14152 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 506564 Cow
    • Entrez Gene: 8661 Human
    • Entrez Gene: 13669 Mouse
    • Entrez Gene: 292148 Rat
    • Entrez Gene: 443632 Xenopus laevis
    • Entrez Gene: 100124719 Xenopus tropicalis
    • Omim: 602039 Human
    • SwissProt: Q14152 Human
    • SwissProt: P23116 Mouse
    • SwissProt: Q1JU68 Rat
    • SwissProt: A2VD00 Xenopus laevis
    • SwissProt: A4II09 Xenopus tropicalis
    • Unigene: 523299 Human
    • Unigene: 713331 Human
    • Unigene: 2238 Mouse
    • Unigene: 482533 Mouse
    • Unigene: 1644 Rat
    • Unigene: 57279 Xenopus laevis
    see all
  • Alternative names

    • centrosomin homolog antibody
    • cytoplasmic protein p167 antibody
    • eIF 3 theta antibody
    • eIF-3-theta antibody
    • EIF3 antibody
    • eIF3 p167 antibody
    • eIF3 p170 antibody
    • eIF3 p180 antibody
    • EIF3 p180 subunit antibody
    • eIF3 p185 antibody
    • eIF3 theta antibody
    • eIF3a antibody
    • EIF3A_HUMAN antibody
    • EIF3S10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit 10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit A antibody
    • OTTHUMP00000020589 antibody
    • P167 antibody
    • p180 antibody
    • p185 antibody
    • TIF32 antibody
    see all

Images

  • Western blot - Anti-eIF3A antibody - N-terminal (ab155412)
    Western blot - Anti-eIF3A antibody - N-terminal (ab155412)
    Anti-eIF3A antibody - N-terminal (ab155412) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 166 kDa



    5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (ab155412)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (ab155412)
    Immunofluorescence analysis of methanol fixed A431 cells, labeling eIF3A with ab155412 at 1/200. Lower image shows costaining with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody - N-terminal (ab155412)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody - N-terminal (ab155412)
    Immunohistochemical analysis of paraffin embedded Hepatoma tissue, labeling eIF3A with ab155412 at 1/500.
  • Western blot - Anti-eIF3A antibody - N-terminal (ab155412)
    Western blot - Anti-eIF3A antibody - N-terminal (ab155412)
    Anti-eIF3A antibody - N-terminal (ab155412) at 1/90000 dilution + 24kDa recombinant eIF3A at 0.1 µg

    Predicted band size: 166 kDa

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab155412? Please let us know so that we can cite the reference in this datasheet.

ab155412 has not yet been referenced specifically in any publications.

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