Anti-eIF3A antibody - N-terminal (ab155412)
- Datasheet
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- Protocols
Overview
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Product name
Anti-eIF3A antibody - N-terminal
See all eIF3A primary antibodies -
Description
Rabbit polyclonal to eIF3A - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 121-347 of Human eIF3A (Uniprot: Q14152)
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Positive control
- 293T; A431; Jurkat or Raji cell lysates, Hepatoma tissue, A431 cells
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General notes
Keep as a concentrated solution
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 20% Glycerol, 78% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab155412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 166 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426). -
Sequence similarities
Belongs to the eIF-3 subunit A family.
Contains 1 PCI domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 506564 Cow
- Entrez Gene: 8661 Human
- Entrez Gene: 13669 Mouse
- Entrez Gene: 292148 Rat
- Entrez Gene: 443632 Xenopus laevis
- Entrez Gene: 100124719 Xenopus tropicalis
- Omim: 602039 Human
- SwissProt: Q14152 Human
see all -
Alternative names
- centrosomin homolog antibody
- cytoplasmic protein p167 antibody
- eIF 3 theta antibody
see all
Images
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Western blot - Anti-eIF3A antibody - N-terminal (ab155412)Anti-eIF3A antibody - N-terminal (ab155412) at 1/500 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 166 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (ab155412)Immunofluorescence analysis of methanol fixed A431 cells, labeling eIF3A with ab155412 at 1/200. Lower image shows costaining with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody - N-terminal (ab155412)Immunohistochemical analysis of paraffin embedded Hepatoma tissue, labeling eIF3A with ab155412 at 1/500. -
Western blot - Anti-eIF3A antibody - N-terminal (ab155412)Anti-eIF3A antibody - N-terminal (ab155412) at 1/90000 dilution + 24kDa recombinant eIF3A at 0.1 µg
Predicted band size: 166 kDa
Protocols
References
ab155412 has not yet been referenced specifically in any publications.
