Key features and details
- Rabbit polyclonal to EIF3C
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-EIF3C antibody
See all EIF3C primary antibodies
DescriptionRabbit polyclonal to EIF3C
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human EIF3C aa 850-913 (C terminal).
- Whole cell lysate from HEK293 (human), C2C12 (mouse), and L6 (Rat) cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/15000 - 1/30000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa).|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Sequence similaritiesBelongs to the eIF-3 subunit C family.
Contains 1 PCI domain.
modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation.
- Information by UniProt
- Cell migration inducing protein 17 antibody
- eIF 3c antibody
- eIF3 p110 antibody
ab19360 at 0.04ab19360 at 0.04µg/ml for Western Blot (A and B) and at 5µg/mg for immunoprecipitation (B), staining (5, 15 or 50µg for Western blot) human HEK293, mouse C2C12 and rat L6 cells (A) and (1mg for immunoprecipitation) whole cell lysate from HEK293 cells (B). Detection was by chemiluminescence with exposure times of two to four minutes.
µg/ml for Western Blot (A and B) and at 5 µg/mg for immunoprecipitation (B), staining (5, 15 or 50 µg for Western blot) human HEK293, mouse C2C12 and rat L6 cells (A) and (1mg for immunoprecipitation) whole cell lysate from HEK293 cells (B). Detection was by chemiluminescence with exposure times of two to four minutes.
ab19360 has not yet been referenced specifically in any publications.