Anti-EIF3C antibody (ab237757)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-EIF3C antibody
See all EIF3C primary antibodies -
Description
Rabbit polyclonal to EIF3C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EIF3C aa 201-500.
Database link: Q99613 -
Positive control
- WB: MCF7, HeLa and A-375 whole cell lysates. IHC-P: Human small intestine tissue. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab237757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/5000. Predicted molecular weight: 105 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
Sequence similarities
Belongs to the eIF-3 subunit C family.
Contains 1 PCI domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8663 Human
- Omim: 603916 Human
- SwissProt: Q99613 Human
- Unigene: 567374 Human
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Alternative names
- Cell migration inducing protein 17 antibody
- eIF 3c antibody
- eIF3 p110 antibody
see all
Images
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Western blot - Anti-EIF3C antibody (ab237757)All lanes : Anti-EIF3C antibody (ab237757) at 1/2000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 105 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3C antibody (ab237757)Paraffin-embedded human small intestine tissue stained for EIF3C using ab237757 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-EIF3C antibody (ab237757)HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for EIF3C using ab237757 at a dilution of 1/100 in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).
References
ab237757 has not yet been referenced specifically in any publications.
