Overview

  • Product name

  • Description

    Rabbit polyclonal to EIF3C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human EIF3C aa 201-500.
    Database link: Q99613

  • Positive control

    • WB: MCF7, HeLa and A-375 whole cell lysates. IHC-P: Human small intestine tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 105 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities

    Belongs to the eIF-3 subunit C family.
    Contains 1 PCI domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell migration inducing protein 17 antibody
    • eIF 3c antibody
    • eIF3 p110 antibody
    • eIF3-p110 antibody
    • eIF3c antibody
    • EIF3C_HUMAN antibody
    • EIF3CL antibody
    • EIF3S8 antibody
    • EIF3S8 protein antibody
    • Eukaryotic translation initiation factor 3 subunit 8 (110kD) antibody
    • Eukaryotic translation initiation factor 3 subunit 8 110kDa antibody
    • Eukaryotic translation initiation factor 3 subunit 8 antibody
    • Eukaryotic translation initiation factor 3 subunit C antibody
    see all

Images

  • All lanes : Anti-EIF3C antibody (ab237757) at 1/2000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 105 kDa

  • Paraffin-embedded human small intestine tissue stained for EIF3C using ab237757 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for EIF3C using ab237757 at a dilution of 1/100 in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).

References

ab237757 has not yet been referenced specifically in any publications.

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