Overview

  • Product name
    Anti-EIF3C antibody - N-terminal
    See all EIF3C primary antibodies
  • Description
    Rabbit polyclonal to EIF3C - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EIF3C aa 126-155 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q99613

  • Positive control
    • IHC-P: Human cerebellum tissue. Flow Cyt: K-562 cells. WB: K-562 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 105 kDa.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities
    Belongs to the eIF-3 subunit C family.
    Contains 1 PCI domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell migration inducing protein 17 antibody
    • eIF 3c antibody
    • eIF3 p110 antibody
    • eIF3-p110 antibody
    • eIF3c antibody
    • EIF3C_HUMAN antibody
    • EIF3CL antibody
    • EIF3S8 antibody
    • EIF3S8 protein antibody
    • Eukaryotic translation initiation factor 3 subunit 8 (110kD) antibody
    • Eukaryotic translation initiation factor 3 subunit 8 110kDa antibody
    • Eukaryotic translation initiation factor 3 subunit 8 antibody
    • Eukaryotic translation initiation factor 3 subunit C antibody
    see all

Images

  • Anti-EIF3C antibody - N-terminal (ab170841) at 1/100 dilution + K-562 cell line lysate at 35 µg

    Predicted band size: 105 kDa

  • Flow cytometric analysis of K-562 cells labeling EIF3C with ab170841 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue labeling EIF3C with ab170841 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

This product has been referenced in:
  • Hao J  et al. Eukaryotic translation initiation factor 3, subunit C is overexpressed and promotes cell proliferation in human glioma U-87 MG cells. Oncol Lett 9:2525-2533 (2015). Read more (PubMed: 26137101) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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