Anti-EIF3D antibody (ab155419)
Key features and details
- Rabbit polyclonal to EIF3D
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EIF3D antibody
See all EIF3D primary antibodies -
Description
Rabbit polyclonal to EIF3D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 241-528 of Human EIF3D (O15371).
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Positive control
- HeLaS3, mouse liver, PC12 and Rat2 whole cell lysates. HUH7 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 64 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
Sequence similarities
Belongs to the eIF-3 subunit D family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8664 Human
- Entrez Gene: 55944 Mouse
- Entrez Gene: 362952 Rat
- Omim: 603915 Human
- SwissProt: O15371 Human
- SwissProt: O70194 Mouse
- SwissProt: Q6AYK8 Rat
- Unigene: 55682 Human
see all -
Alternative names
- eIF 3 zeta antibody
- eIF-3-zeta antibody
- eIF3 p66 antibody
see all
Images
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Anti-EIF3D antibody (ab155419) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 64 kDa
7.5 % SDS-PAGE -
Anti-EIF3D antibody (ab155419) at 1/5000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 64 kDa
7.5 % SDS-PAGE -
All lanes : Anti-EIF3D antibody (ab155419) at 1/3000 dilution
Lane 1 : PC12 whole cell lysate
Lane 2 : Rat2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 64 kDa
7.5 % SDS-PAGE -
Immunohistochemical analysis of paraffin embedded HUH7 xenograft labeling EIF3D with ab155419 antibody at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab155419 has been referenced in 9 publications.
- Zhong Y & Lan J Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78. Bioengineered 13:1952-1961 (2022). WB, IHC-P ; Human . PubMed: 35104170
- Theodoridis PR et al. Local translation in nuclear condensate amyloid bodies. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33568529
- Pan Y et al. Reduced eIF3d accelerates HIV disease progression by attenuating CD8+ T cell function. J Transl Med 17:167 (2019). PubMed: 31118081
- He J et al. High expression of eIF3d is associated with poor prognosis in patients with gastric cancer. Cancer Manag Res 9:539-544 (2017). PubMed: 29123423
- Thakor N et al. Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation. RNA Biol 14:553-567 (2017). PubMed: 26828225
- Pan XW et al. EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling. Int J Oncol 48:2580-90 (2016). PubMed: 27035563
- Lin Y et al. Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer. FEBS Open Bio 6:1201-1210 (2016). WB, IHC . PubMed: 28203520
- Ren M et al. RNAi-Mediated Silencing of EIF3D Alleviates Proliferation and Migration of Glioma U251 and U87MG Cells. Chem Biol Drug Des 86:715-22 (2015). PubMed: 25682860
- Yu X et al. Lentivirus-mediated knockdown of eukaryotic translation initiation factor 3 subunit D inhibits proliferation of HCT116 colon cancer cells. Biosci Rep 34:e00161 (2014). WB . PubMed: 25370813