Product nameAnti-EIF3D antibody
See all EIF3D primary antibodies
DescriptionRabbit polyclonal to EIF3D
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Synthetic peptide within Human EIF3D aa 150-200. The exact sequence is proprietary.
Database link: O15371
- WB: HEK-293T whole cell lysate; IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Sequence similaritiesBelongs to the eIF-3 subunit D family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- eIF 3 zeta antibody
- eIF-3-zeta antibody
- eIF3 p66 antibody
All lanes : Anti-EIF3D antibody (ab264228) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293-T whole cell lysate at 50 µg
Detected with chemiluminescence with exposure time of 3 minutes.
EIF3D was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264228 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264228 at 0.4 µg/ml.
Lane 1: ab264228 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264228 has not yet been referenced specifically in any publications.