Overview

  • Product name

    Anti-eIF3e antibody [EPR6877]
    See all eIF3e primary antibodies
  • Description

    Rabbit monoclonal [EPR6877] to eIF3e
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF3e aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Jurkat, 293T, BxPC-3, A431, Saos 2, HepG2 and NIH 3T3 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 9.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6877
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
    • Tissue specificity

      Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
    • Sequence similarities

      Belongs to the eIF-3 subunit E family.
      Contains 1 PCI domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus > PML body.
    • Information by UniProt
    • Database links

    • Alternative names

      • eIF-3 p48 antibody
      • eIF3e antibody
      • EIF3E_HUMAN antibody
      • EIF3S6 antibody
      • eIFe antibody
      • Eukaryotic translation initiation factor 3 subunit 6 antibody
      • Eukaryotic translation initiation factor 3 subunit E antibody
      • eukaryotic translation initiation factor 3, subunit 6 (48kD) antibody
      • INT6 antibody
      • mammary tumor-associated protein INT6 antibody
      • murine mammary tumor integration site 6 (oncogene homolog) antibody
      • Viral integration site protein INT-6 homolog antibody
      see all

    Images

    • All lanes : Anti-eIF3e antibody [EPR6877] (ab125033) at 1/1000 dilution

      Lanes 1 & 6 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : BxPC3 cell lysate
      Lane 4 : A431 cell lysate
      Lane 5 : Saos 2 cell lysate
      Lane 7 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab125033.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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