Product nameAnti-eIF3e antibody [EPR6878(2)]
See all eIF3e primary antibodies
DescriptionRabbit monoclonal [EPR6878(2)] to eIF3e
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human eIF3e aa 250-350. The exact sequence is proprietary.
- Jurkat, 293T, BxPC3 and Saos 2 cell lysates; Human brain tissue; 293T cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Tissue specificityUbiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
Sequence similaritiesBelongs to the eIF-3 subunit E family.
Contains 1 PCI domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus > PML body.
- Information by UniProt
- eIF-3 p48 antibody
- eIF3e antibody
- EIF3E_HUMAN antibody
All lanes : Anti-eIF3e antibody [EPR6878(2)] (ab134958) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : Saos 2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling eIF3e with ab134958 at 1/50 dilution.
Immunofluorescent analysis of 293T cells labelling eIF3e with ab134958 at 1/250 dilution.
ab134958 has not yet been referenced specifically in any publications.