Overview

  • Product name

    Anti-eIF3e antibody [EPR6878(2)]
    See all eIF3e primary antibodies
  • Description

    Rabbit monoclonal [EPR6878(2)] to eIF3e
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF3e aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Jurkat, 293T, BxPC3 and Saos 2 cell lysates; Human brain tissue; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6878(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
    • Tissue specificity

      Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
    • Sequence similarities

      Belongs to the eIF-3 subunit E family.
      Contains 1 PCI domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus > PML body.
    • Information by UniProt
    • Database links

    • Alternative names

      • eIF-3 p48 antibody
      • eIF3e antibody
      • EIF3E_HUMAN antibody
      • EIF3S6 antibody
      • eIFe antibody
      • Eukaryotic translation initiation factor 3 subunit 6 antibody
      • Eukaryotic translation initiation factor 3 subunit E antibody
      • eukaryotic translation initiation factor 3, subunit 6 (48kD) antibody
      • INT6 antibody
      • mammary tumor-associated protein INT6 antibody
      • murine mammary tumor integration site 6 (oncogene homolog) antibody
      • Viral integration site protein INT-6 homolog antibody
      see all

    Images

    • All lanes : Anti-eIF3e antibody [EPR6878(2)] (ab134958) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : BxPC3 cell lysate
      Lane 4 : Saos 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling eIF3e with ab134958 at 1/50 dilution.
    • Immunofluorescent analysis of 293T cells labelling eIF3e with ab134958 at 1/250 dilution.

    References

    ab134958 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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