Anti-EIF3F antibody (ab176853)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
Anti-EIF3F antibody
See all EIF3F primary antibodies -
Description
Rabbit polyclonal to EIF3F -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
-
Immunogen
Synthetic peptide within Human EIF3F aa 307-357 (C terminal). The exact sequence is proprietary. NP_003745.1
Sequence:FLMS LVNQVPKIVP DDFETMLNSN INDLLMVTYL ANLTQSQIAL NEKLVNL
Database link: O00303 -
Positive control
- HeLa and NIH 3T3 whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176853 was affinity purified using an epitope specific to EIF3F immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab176853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 38 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
-
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. -
Sequence similarities
Belongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain. -
Domain
The MPN domain mediates deubiquitinating activity. -
Post-translational
modificationsPhosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 451007 Chimpanzee
- Entrez Gene: 513793 Cow
- Entrez Gene: 476840 Dog
- Entrez Gene: 100733279 Guinea pig
- Entrez Gene: 8665 Human
- Entrez Gene: 66085 Mouse
- Entrez Gene: 293427 Rat
- Entrez Gene: 706269 Rhesus monkey
see all -
Alternative names
- Deubiquitinating enzyme eIF3f antibody
- eIF 3 epsilon antibody
- eIF-3-epsilon antibody
see all
Images
-
Western blot - Anti-EIF3F antibody (ab176853)All lanes : Anti-EIF3F antibody (ab176853) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-EIF3F antibody (ab176853)Detection of EIF3F in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176853 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab176853 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References
This product has been referenced in:
- V'kovski P et al. Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling. Elife 8:N/A (2019). Read more (PubMed: 30632963) »
- Son YH et al. Dexamethasone downregulates caveolin-1 causing muscle atrophy via inhibited insulin signaling. J Endocrinol 225:27-37 (2015). WB ; Mouse . Read more (PubMed: 25688118) »
