• Product name

  • Description

    Rabbit polyclonal to EIF3F
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal sequence of human EIF3F.

  • Positive control

    • Human brain tissue. Extracts from Jurkat cells and COLO205 cells.



Our Abpromise guarantee covers the use of ab74568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/40000.


  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
  • Sequence similarities

    Belongs to the eIF-3 subunit F family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain

    The MPN domain mediates deubiquitinating activity.
  • Post-translational

    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme eIF3f antibody
    • eIF 3 epsilon antibody
    • eIF-3-epsilon antibody
    • eIF3 p47 antibody
    • eIF3 p47 subunit antibody
    • eIF3-epsilon antibody
    • eIF3-p47 antibody
    • eIF3f antibody
    • EIF3F_HUMAN antibody
    • EIF3S5 antibody
    • EIF3S5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit 5 antibody
    • Eukaryotic translation initiation factor 3 subunit 5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit F antibody
    • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) antibody
    • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa antibody
    • eukaryotic translation initiation factor 3, subunit F antibody
    • translation initiation factor 3 47 kda subunit antibody
    see all


  • All lanes : Anti-EIF3F antibody (ab74568) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from COLO205 cells
    Lane 3 : Extracts from COLO205 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunohistochemistry analysis of EIF3F in paraffin-embedded human brain tissue using ab74568, at 1/50-1/100 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.


This product has been referenced in:

  • Song Z  et al. Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle. Sci Rep 7:5028 (2017). Read more (PubMed: 28694500) »
  • Villa N  et al. Human eukaryotic initiation factor 4G (eIF4G) protein binds to eIF3c, -d, and -e to promote mRNA recruitment to the ribosome. J Biol Chem 288:32932-40 (2013). WB ; Human . Read more (PubMed: 24092755) »
See all 2 Publications for this product

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