Key features and details
- Rabbit polyclonal to EIF3F
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EIF3F antibody
See all EIF3F primary antibodies
DescriptionRabbit polyclonal to EIF3F
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from internal sequence of human EIF3F.
- Human brain tissue. Extracts from Jurkat cells and COLO205 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 38 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
Sequence similaritiesBelongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain.
DomainThe MPN domain mediates deubiquitinating activity.
modificationsPhosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
- Information by UniProt
- Deubiquitinating enzyme eIF3f antibody
- eIF 3 epsilon antibody
- eIF-3-epsilon antibody
All lanes : Anti-EIF3F antibody (ab74568) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from COLO205 cells
Lane 3 : Extracts from COLO205 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Immunohistochemistry analysis of EIF3F in paraffin-embedded human brain tissue using ab74568, at 1/50-1/100 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
ab74568 has been referenced in 2 publications.
- Song Z et al. Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle. Sci Rep 7:5028 (2017). PubMed: 28694500
- Villa N et al. Human eukaryotic initiation factor 4G (eIF4G) protein binds to eIF3c, -d, and -e to promote mRNA recruitment to the ribosome. J Biol Chem 288:32932-40 (2013). WB ; Human . PubMed: 24092755