Recombinant Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16146] to eIF3g - N-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF3g antibody [EPR16146] - N-terminal
See all eIF3g primary antibodies -
Description
Rabbit monoclonal [EPR16146] to eIF3g - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, Molt-4, HepG2, K562, PC-12 and NIH/3T3 cell lysate. Human colonic carcinoma tissue. Rat skeletal muscle tissue. HepG2 cells. 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16146 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/80.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/150.
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Notes |
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Flow Cyt (Intra)
1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/150. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA. -
Sequence similarities
Belongs to the eIF-3 subunit G family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 8666 Human
- Entrez Gene: 53356 Mouse
- Entrez Gene: 298700 Rat
- Omim: 603913 Human
- SwissProt: O75821 Human
- SwissProt: Q9Z1D1 Mouse
- SwissProt: Q5RK09 Rat
- Unigene: 529059 Human
see all -
Alternative names
- 44kDa antibody
- Anti EIF3S4 antibody
- eIF 3 delta antibody
see all
Images
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All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/1000 dilution
Lane 1 : PC-12 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded rat skeletal muscle tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling eIF3g using ab191422 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100. The two negative controls: Primary ab concentration (anti-EIF3G), Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
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Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed 293T cells labeling eIF3g using ab191422 at a 1/80 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191422 has not yet been referenced specifically in any publications.