Recombinant
RabMAb

Recombinant Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)

Overview

  • Product name

    Anti-eIF3g antibody [EPR16146] - N-terminal
    See all eIF3g primary antibodies
  • Description

    Rabbit monoclonal [EPR16146] to eIF3g - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF3g aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O75821

  • Positive control

    • 293T, Molt-4, HepG2, K562, PC-12 and NIH/3T3 cell lysate. Human colonic carcinoma tissue. Rat skeletal muscle tissue. HepG2 cells. 293T cells.
  • General notes

     

     

     This product was previously labelled as EIF3S4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/150.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
  • Sequence similarities

    Belongs to the eIF-3 subunit G family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • 44kDa antibody
    • Anti EIF3S4 antibody
    • eIF 3 delta antibody
    • eIF 3 RNA binding subunit antibody
    • eIF-3 RNA-binding subunit antibody
    • eIF-3-delta antibody
    • eIF3 delta antibody
    • eIF3 p42 antibody
    • eIF3 p44 antibody
    • eIF3g antibody
    • EIF3G_HUMAN antibody
    • EIF3S4 antibody
    • EIF3S4 antibody antibody
    • EIF3S4, formerly antibody
    • Eukaryotic translation initiation factor 3 antibody
    • Eukaryotic translation initiation factor 3 RNA binding subunit antibody
    • Eukaryotic translation initiation factor 3 RNA-binding subunit antibody
    • Eukaryotic translation initiation factor 3 subunit 4 antibody
    • Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody
    • Eukaryotic translation initiation factor 3 subunit G antibody
    • Eukaryotic translation initiation factor 3 subunit p42 antibody
    • Eukaryotic translation initiation factor 3, subunit 4, formerly antibody
    • Subunit 4 (delta 44kD) antibody
    • Subunit 4 delta antibody
    see all

Images

  • All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Molt-4 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded rat skeletal muscle tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling eIF3g using ab191422 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100. The two negative controls: Primary ab concentration (anti-EIF3G), Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.

  • Flow Cytometry analysis of 2% paraformaldehyde fixed 293T cells labeling eIF3g using ab191422 at a 1/80 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green).

References

ab191422 has not yet been referenced specifically in any publications.

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