Overview

  • Product name

    Anti-eIF3g antibody [EPR16147]
    See all eIF3g primary antibodies
  • Description

    Rabbit monoclonal [EPR16147] to eIF3g
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human eIF3g aa 200-300. The exact sequence is proprietary.
    Database link: O75821

  • Positive control

    • 293T, MOLT4, K562, mouse Raw 264.7, mouse NIH 3T3 and rat PC12 cell lysates. HeLa cells.
  • General notes

     

     

     This product was previously labelled as EIF3S4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/100.
IP 1/40.

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
  • Sequence similarities

    Belongs to the eIF-3 subunit G family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • 44kDa antibody
    • Anti EIF3S4 antibody
    • eIF 3 delta antibody
    • eIF 3 RNA binding subunit antibody
    • eIF-3 RNA-binding subunit antibody
    • eIF-3-delta antibody
    • eIF3 delta antibody
    • eIF3 p42 antibody
    • eIF3 p44 antibody
    • eIF3g antibody
    • EIF3G_HUMAN antibody
    • EIF3S4 antibody
    • EIF3S4 antibody antibody
    • EIF3S4, formerly antibody
    • Eukaryotic translation initiation factor 3 antibody
    • Eukaryotic translation initiation factor 3 RNA binding subunit antibody
    • Eukaryotic translation initiation factor 3 RNA-binding subunit antibody
    • Eukaryotic translation initiation factor 3 subunit 4 antibody
    • Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody
    • Eukaryotic translation initiation factor 3 subunit G antibody
    • Eukaryotic translation initiation factor 3 subunit p42 antibody
    • Eukaryotic translation initiation factor 3, subunit 4, formerly antibody
    • Subunit 4 (delta 44kD) antibody
    • Subunit 4 delta antibody
    see all

Images

  • All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution

    Lane 1 : mouse Raw 264.7 cell lysate
    Lane 2 : rat PC12 cell lysate
    Lane 3 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling eIF3g with ab192601 at a 1/100 dilution ( 7.0 μg/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.

  • Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling eIF3g using ab192601 at 1/40 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.

References

ab192601 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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