Overview

  • Product name

  • Description

    Rabbit polyclonal to EIF3H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment corresponding to Human EIF3H (internal sequence).
    Database link: O15372

  • Positive control

    • WB: A431, NCI-H1299 and HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities

    Belongs to the eIF-3 subunit H family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • eIF-3-gamma antibody
    • eIF3 gamma antibody
    • eIF3 p40 antibody
    • eIF3 p40 subunit antibody
    • eIF3h antibody
    • EIF3H_HUMAN antibody
    • EIF3S3 antibody
    • Eukaryotic translation initiation factor 3 subunit 3 antibody
    • Eukaryotic translation initiation factor 3 subunit H antibody
    • MGC102958 antibody
    see all

Images

  • All lanes : Anti-EIF3H antibody (ab228536) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1229 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa

References

ab228536 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse embryonic stem cells (J1)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 02 2018

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