Overview

  • Product name
    Anti-EIF3H antibody - N-terminal
    See all EIF3H primary antibodies
  • Description
    Rabbit polyclonal to EIF3H - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EIF3H aa 69-99 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O15372

  • Positive control
    • WB: 293 cell lysates transiently transfected with EIF3H. IHC-P: Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab171419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities
    Belongs to the eIF-3 subunit H family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-3-gamma antibody
    • eIF3 gamma antibody
    • eIF3 p40 antibody
    • eIF3 p40 subunit antibody
    • eIF3h antibody
    • EIF3H_HUMAN antibody
    • EIF3S3 antibody
    • Eukaryotic translation initiation factor 3 subunit 3 antibody
    • Eukaryotic translation initiation factor 3 subunit H antibody
    • MGC102958 antibody
    see all

Images

  • All lanes : Anti-EIF3H antibody - N-terminal (ab171419) at 1/100 dilution

    Lane 1 : 293 cell lysate transiently nontransfected
    Lane 2 : 293 cell lysate transiently transfected with EIF3H

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 40 kDa

  • Immunohistochemical analysis of paraffin embedded Human hepatocarcinoma tissue labeling EIF3H with ab171419 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171419 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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