Anti-eIF3K antibody [2313C2a] (ab50736)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-eIF3K antibody [2313C2a]
See all eIF3K primary antibodies -
DescriptionMouse monoclonal [2313C2a] to eIF3K
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Host speciesMouse
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Tested applicationsSuitable for: WB, Dot blotmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant human EIF3S12
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Positive control
- Recombinant human EIF3S12
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading... -
PurityProtein G purified
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Purification notesab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
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ClonalityMonoclonal
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Clone number2313C2a
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab50736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. | |
| Dot blot | Use at an assay dependent dilution. |
Target
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FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
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Tissue specificityUbiquitous, with the highest levels of expression in brain, testis and kidney.
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Sequence similaritiesBelongs to the eIF-3 subunit K family.
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Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 27335 Human
- Omim: 609596 Human
- SwissProt: Q9UBQ5 Human
- Unigene: 314359 Human
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Alternative names
- ARG134 antibody
- eIF 3 p25 antibody
- eIF 3 p28 antibody
see all
Images
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![Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736.bmp)
Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein
Predicted band size: 25 kDa
Observed band size: 33 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . Read more (PubMed: 19023046) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"