Anti-eIF3K antibody [2313C2a] (ab50736)
![Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736-414932-anti-eif3k-antibody-2313c2a-western-blot-human-knockout-lysate.jpg "Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)")
Key features and details
- Mouse monoclonal [2313C2a] to eIF3K
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-eIF3K antibody [2313C2a]
See all eIF3K primary antibodies -
Description
Mouse monoclonal [2313C2a] to eIF3K -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human EIF3S12
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Positive control
- WB: HEK293 and THP1 cell lysates; Recombinant human EIF3S12.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2313C2a -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab50736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
Tissue specificity
Ubiquitous, with the highest levels of expression in brain, testis and kidney. -
Sequence similarities
Belongs to the eIF-3 subunit K family. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 27335 Human
- Omim: 609596 Human
- SwissProt: Q9UBQ5 Human
- Unigene: 314359 Human
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Alternative names
- ARG134 antibody
- eIF 3 p25 antibody
- eIF 3 p28 antibody
see all
Images
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Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)All lanes : Anti-eIF3K antibody [2313C2a] (ab50736) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : THP1 (Human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaLanes 1-3: Merged signal (red and green). Green - ab50736 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa.
ab50736 Anti-eIF3K antibody [2313C2a] was shown to specifically react with eIF3K in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266841 (knockout cell lysate ab258857) was used. Wild-type and eIF3K knockout samples were subjected to SDS-PAGE. ab50736 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736.bmp)
Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein
Predicted band size: 25 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (1)
ab50736 has been referenced in 1 publication.
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046
