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Validated using a knockout cell line

Anti-eIF3K antibody [2313C2a] (ab50736)

  • Datasheet
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Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
  • Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)

Key features and details

  • Mouse monoclonal [2313C2a] to eIF3K
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2b

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Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Knockout
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Human EIF3K knockout HEK293T cell line (ab266841)
Protein
Product image
Recombinant Human eIF3K protein (ab80036)

View more associated products

Overview

  • Product name

    Anti-eIF3K antibody [2313C2a]
    See all eIF3K primary antibodies
  • Description

    Mouse monoclonal [2313C2a] to eIF3K
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant human EIF3S12

  • Positive control

    • WB: HEK293 and THP1 cell lysates; Recombinant human EIF3S12.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    2313C2a
  • Isotype

    IgG2b
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Serotonin Receptors
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • KO cell lines

    • Human EIF3K knockout HEK293T cell line (ab266841)
  • KO cell lysates

    • Human EIF3K knockout HEK293T cell lysate (ab258857)
  • Recombinant Protein

    • Recombinant Human eIF3K protein (ab80036)

Applications

Our Abpromise guarantee covers the use of ab50736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Tissue specificity

    Ubiquitous, with the highest levels of expression in brain, testis and kidney.
  • Sequence similarities

    Belongs to the eIF-3 subunit K family.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession Q9UBQ5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27335 Human
    • Omim: 609596 Human
    • SwissProt: Q9UBQ5 Human
    • Unigene: 314359 Human
    • Alternative names

      • ARG134 antibody
      • eIF 3 p25 antibody
      • eIF 3 p28 antibody
      • eIF-3 p25 antibody
      • eIF-3 p28 antibody
      • EIF3-p28 antibody
      • eif3k antibody
      • EIF3K_HUMAN antibody
      • EIF3S12 antibody
      • Eukaryotic translation initiation factor 3 subunit 12 antibody
      • Eukaryotic translation initiation factor 3 subunit K antibody
      • HSPC029 antibody
      • M9 antibody
      • MSTP001 antibody
      • Muscle specific gene M9 protein antibody
      • Muscle-specific gene M9 protein antibody
      • PLAC 24 antibody
      • PLAC-24 antibody
      • PLAC24 antibody
      • PRO1474 antibody
      • PTD001 antibody
      see all

    Images

    • Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      All lanes : Anti-eIF3K antibody [2313C2a] (ab50736) at 1/500 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : THP1 (Human monocytic leukemia cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab50736 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa.

       ab50736 Anti-eIF3K antibody [2313C2a]  was shown to specifically react with eIF3K in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266841 (knockout cell lysate ab258857) was used. Wild-type and eIF3K knockout samples were subjected to SDS-PAGE. ab50736 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein

      Predicted band size: 25 kDa
      Observed band size: 33 kDa
      why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab50736? Please let us know so that we can cite the reference in this datasheet.

    ab50736 has been referenced in 1 publication.

    • Cohen AA  et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046

    Customer reviews and Q&As

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