Anti-eIF3K antibody [2313C2a] (ab50736)
![Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736-414932-anti-eif3k-antibody-2313c2a-western-blot-human-knockout-lysate.jpg "Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)")
Key features and details
- Mouse monoclonal [2313C2a] to eIF3K
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-eIF3K antibody [2313C2a]
See all eIF3K primary antibodies -
Description
Mouse monoclonal [2313C2a] to eIF3K -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant human EIF3S12
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Positive control
- WB: HEK293 and THP1 cell lysates; Recombinant human EIF3S12.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2313C2a -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
Tissue specificity
Ubiquitous, with the highest levels of expression in brain, testis and kidney. -
Sequence similarities
Belongs to the eIF-3 subunit K family. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 27335 Human
- Omim: 609596 Human
- SwissProt: Q9UBQ5 Human
- Unigene: 314359 Human
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Alternative names
- ARG134 antibody
- eIF 3 p25 antibody
- eIF 3 p28 antibody
see all
Images
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Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)All lanes : Anti-eIF3K antibody [2313C2a] (ab50736) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : THP1 (Human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaLanes 1-3: Merged signal (red and green). Green - ab50736 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa.
ab50736 Anti-eIF3K antibody [2313C2a] was shown to specifically react with eIF3K in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266841 (knockout cell lysate ab258857) was used. Wild-type and eIF3K knockout samples were subjected to SDS-PAGE. ab50736 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736.bmp)
Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein
Predicted band size: 25 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab50736 has been referenced in 1 publication.
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046
