Anti-eIF3K antibody [2313C2a] (ab50736)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-eIF3K antibody [2313C2a]
See all eIF3K primary antibodies -
Description
Mouse monoclonal [2313C2a] to eIF3K -
Host species
Mouse -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human EIF3S12
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Positive control
- Recombinant human EIF3S12
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2313C2a -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab50736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Predicted molecular weight: 25 kDa. | |
| Dot blot | Use at an assay dependent dilution. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
Tissue specificity
Ubiquitous, with the highest levels of expression in brain, testis and kidney. -
Sequence similarities
Belongs to the eIF-3 subunit K family. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 27335 Human
- Omim: 609596 Human
- SwissProt: Q9UBQ5 Human
- Unigene: 314359 Human
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Alternative names
- ARG134 antibody
- eIF 3 p25 antibody
- eIF 3 p28 antibody
see all
Images
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![Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736.bmp)
Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein
Predicted band size: 25 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . Read more (PubMed: 19023046) »
