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  1. Link

    eif3k-antibody-2313c2a-ab50736.pdf

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Validated using a knockout cell line

Anti-eIF3K antibody [2313C2a] (ab50736)

  • Datasheet
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Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
  • Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)

Key features and details

  • Mouse monoclonal [2313C2a] to eIF3K
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

You may also be interested in

Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Knockout
Product image
Human EIF3K knockout HEK-293T cell line (ab266841)
Protein
Product image
Recombinant Human eIF3K protein (ab80036)

View more associated products

Overview

  • Product name

    Anti-eIF3K antibody [2313C2a]
    See all eIF3K primary antibodies
  • Description

    Mouse monoclonal [2313C2a] to eIF3K
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant human EIF3S12

  • Positive control

    • WB: HEK293 and THP1 cell lysates; Recombinant human EIF3S12.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    2313C2a
  • Isotype

    IgG2b
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Serotonin Receptors
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • KO cell lines

    • Human EIF3K knockout HEK-293T cell line (ab266841)
  • KO cell lysates

    • Human EIF3K knockout HEK-293T cell lysate (ab258857)
  • Recombinant Protein

    • Recombinant Human eIF3K protein (ab80036)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab50736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Tissue specificity

    Ubiquitous, with the highest levels of expression in brain, testis and kidney.
  • Sequence similarities

    Belongs to the eIF-3 subunit K family.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession Q9UBQ5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27335 Human
    • Omim: 609596 Human
    • SwissProt: Q9UBQ5 Human
    • Unigene: 314359 Human
    • Alternative names

      • ARG134 antibody
      • eIF 3 p25 antibody
      • eIF 3 p28 antibody
      • eIF-3 p25 antibody
      • eIF-3 p28 antibody
      • EIF3-p28 antibody
      • eif3k antibody
      • EIF3K_HUMAN antibody
      • EIF3S12 antibody
      • Eukaryotic translation initiation factor 3 subunit 12 antibody
      • Eukaryotic translation initiation factor 3 subunit K antibody
      • HSPC029 antibody
      • M9 antibody
      • MSTP001 antibody
      • Muscle specific gene M9 protein antibody
      • Muscle-specific gene M9 protein antibody
      • PLAC 24 antibody
      • PLAC-24 antibody
      • PLAC24 antibody
      • PRO1474 antibody
      • PTD001 antibody
      see all

    Images

    • Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      All lanes : Anti-eIF3K antibody [2313C2a] (ab50736) at 1/500 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : THP1 (Human monocytic leukemia cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab50736 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa.

       ab50736 Anti-eIF3K antibody [2313C2a]  was shown to specifically react with eIF3K in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266841 (knockout cell lysate ab258857) was used. Wild-type and eIF3K knockout samples were subjected to SDS-PAGE. ab50736 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)
      Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein

      Predicted band size: 25 kDa
      Observed band size: 33 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab50736? Please let us know so that we can cite the reference in this datasheet.

    ab50736 has been referenced in 1 publication.

    • Cohen AA  et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046

    Customer reviews and Q&As

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