Anti-eIF3K antibody (ab85968)
Key features and details
- Rabbit polyclonal to eIF3K
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF3K antibody
See all eIF3K primary antibodies -
Description
Rabbit polyclonal to eIF3K -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide designed within residues: SQLKVWMSKY GWSADESGQI FICSQEE SIKPKNIVE KIDFDSVSSI MASSQ, corresponding to C terminal amino acids 168-218 of Human eIF3K (NP_037366.1)
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Positive control
- Whole cell lysates from HeLa or 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 25 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
Tissue specificity
Ubiquitous, with the highest levels of expression in brain, testis and kidney. -
Sequence similarities
Belongs to the eIF-3 subunit K family. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 515326 Cow
- Entrez Gene: 27335 Human
- Entrez Gene: 73830 Mouse
- Entrez Gene: 292762 Rat
- Omim: 609596 Human
- SwissProt: Q3T0V3 Cow
- SwissProt: Q9UBQ5 Human
- SwissProt: Q9DBZ5 Mouse
see all -
Alternative names
- ARG134 antibody
- eIF 3 p25 antibody
- eIF 3 p28 antibody
see all
Images
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All lanes : Anti-eIF3K antibody (ab85968) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 25 kDa
Exposure time: 10 seconds -
1 mg of HeLa whole cell lysate was immunoprecipitated using 3µg ab85968. Subsequently, 20% of IP was loaded per lane and probed with ab85968 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85968 has not yet been referenced specifically in any publications.