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    eif3l-antibody-ab154527.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-eIF3l antibody (ab154527)

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  • SDS
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Western blot - Anti-eIF3l antibody (ab154527)

    Key features and details

    • Rabbit polyclonal to eIF3l
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-eIF3l antibody
      See all eIF3l primary antibodies
    • Description

      Rabbit polyclonal to eIF3l
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Cow, Human, Xenopus laevis, Xenopus tropicalis
    • Immunogen

      Recombinant fragment corresponding to Human eIF3l aa 330-564.
      Database link: Q9Y262

    • Positive control

      • Mouse brown adipose and white adipose whole cell lysates.
    • General notes

       This product was previously labelled as IF3EI

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • Translation
      • Regulation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Mouse adipose normal tissue lysate - total protein (ab29417)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab154527 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 66 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 66 kDa.

    Target

    • Function

      Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    • Sequence similarities

      Belongs to the eIF-3 subunit L family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q9Y262 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 514449 Cow
      • Entrez Gene: 51386 Human
      • Entrez Gene: 223691 Mouse
      • Entrez Gene: 380037 Xenopus laevis
      • Entrez Gene: 394577 Xenopus tropicalis
      • SwissProt: Q3ZCK1 Cow
      • SwissProt: Q9Y262 Human
      • SwissProt: Q8QZY1 Mouse
      • SwissProt: Q8AVJ0 Xenopus laevis
      • SwissProt: Q6P878 Xenopus tropicalis
      • Unigene: 446852 Human
      • Unigene: 206404 Mouse
      • Unigene: 3626 Xenopus laevis
      see all
    • Alternative names

      • eIEF associated protein HSPC021 antibody
      • EIF3EIP antibody
      • eif3l antibody
      • EIF3L_HUMAN antibody
      • EIF3S11 antibody
      • EIF3S6IP antibody
      • Eukaryotic translation initiation factor 3 subunit 6 interacting antibody
      • Eukaryotic translation initiation factor 3 subunit 6-interacting protein antibody
      • Eukaryotic translation initiation factor 3 subunit E-interacting protein antibody
      • Eukaryotic translation initiation factor 3 subunit L antibody
      • eukaryotic translation initiation factor 3, subunit E interacting antibody
      • HSPC021 antibody
      • HSPC025 antibody
      • MSTP005 antibody
      see all

    Images

    • Western blot - Anti-eIF3l antibody (ab154527)
      Western blot - Anti-eIF3l antibody (ab154527)
      All lanes : Anti-eIF3l antibody (ab154527) at 1/1000 dilution

      Lane 1 : Mouse brown adipose whole cell lysate
      Lane 2 : Mouse white adipose whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 66 kDa



      7.5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab154527? Please let us know so that we can cite the reference in this datasheet.

    ab154527 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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