Anti-eIF3l antibody (ab154527)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-eIF3l antibody
See all eIF3l primary antibodies -
Description
Rabbit polyclonal to eIF3l -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Cow, Human, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human eIF3l aa 330-564.
Database link: Q9Y262 -
Positive control
- Mouse brown adipose and white adipose whole cell lysates.
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General notes
This product was previously labelled as IF3EI
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 66 kDa. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
Sequence similarities
Belongs to the eIF-3 subunit L family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 514449 Cow
- Entrez Gene: 51386 Human
- Entrez Gene: 223691 Mouse
- Entrez Gene: 380037 Xenopus laevis
- Entrez Gene: 394577 Xenopus tropicalis
- SwissProt: Q3ZCK1 Cow
- SwissProt: Q9Y262 Human
- SwissProt: Q8QZY1 Mouse
see all -
Alternative names
- eIEF associated protein HSPC021 antibody
- EIF3EIP antibody
- eif3l antibody
see all
Images
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Western blot - Anti-eIF3l antibody (ab154527)All lanes : Anti-eIF3l antibody (ab154527) at 1/1000 dilution
Lane 1 : Mouse brown adipose whole cell lysate
Lane 2 : Mouse white adipose whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
7.5% SDS PAGE
Protocols
References
ab154527 has not yet been referenced specifically in any publications.
