Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF3l
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide within Human eIF3l aa 514-564. The exact sequence is proprietary.
    Database link: Q9Y262

  • Positive control

    • WB: HeLa, HEK-392T, Jurkat, TCMK-1 and NIH/3T3 cell lysate. IP: eIF3l IP in HEK-293T cell lysate.
  • General notes

     This product was previously labelled as IF3EI

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to EIF3L immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities

    Belongs to the eIF-3 subunit L family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • eIEF associated protein HSPC021 antibody
    • EIF3EIP antibody
    • eif3l antibody
    • EIF3L_HUMAN antibody
    • EIF3S11 antibody
    • EIF3S6IP antibody
    • Eukaryotic translation initiation factor 3 subunit 6 interacting antibody
    • Eukaryotic translation initiation factor 3 subunit 6-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit E-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit L antibody
    • eukaryotic translation initiation factor 3, subunit E interacting antibody
    • HSPC021 antibody
    • HSPC025 antibody
    • MSTP005 antibody
    see all

Images

  • All lanes : Anti-eIF3l antibody (ab241344) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 3 minutes
  • eIF3l was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241344 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241344 at 0.4 µg/ml.

    Lane 1: ab241344 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysate prepared using NETN lysis buffer.

References

ab241344 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241344.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up