Key features and details
- Chicken polyclonal to EIF3S2
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgY
Product nameAnti-EIF3S2 antibody
See all EIF3S2 primary antibodies
DescriptionChicken polyclonal to EIF3S2
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to N terminal amino acids 1-230 of Human EIF3S2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
|ELISA||Use a concentration of 1 µg/ml.|
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Sequence similaritiesBelongs to the eIF-3 subunit I family.
Contains 5 WD repeats.
modificationsPhosphorylated by TGF-beta type II receptor.
- Information by UniProt
- eIF-3-beta antibody
- eIF3 beta antibody
- eIF3 p36 antibody
Direct ELISA test. Serial dilutions of Human EIF3S2 protein were used as test antigen. ab37526 was used as test antibody at 1µg/ml. Goat anti-IgY-HRP was used as secondary antibody.
Anti-EIF3S2 antibody (ab37526) at 1/2000 dilution + E. coli derived fusion protein at 2.5 µg
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
ab37526 has been referenced in 1 publication.
- Angelucci S et al. Proteome analysis of human Wharton's jelly cells during in vitro expansion. Proteome Sci 8:18 (2010). WB ; Human . PubMed: 20346146