• Product name

  • Description

    Rabbit polyclonal to EIF3S2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EIF3S2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41639.)

  • Positive control

    • ab40745 gave a positive signal in the following human whole cell lysates: HeLa, Jurkat, HEK293, HepG2, MCF-7 and SHSY-5Y.



Our Abpromise guarantee covers the use of ab40745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).


  • Function

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities

    Belongs to the eIF-3 subunit I family.
    Contains 5 WD repeats.
  • Post-translational

    Phosphorylated by TGF-beta type II receptor.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • eIF-3-beta antibody
    • eIF3 beta antibody
    • eIF3 p36 antibody
    • eIF3-beta antibody
    • eIF3-p36 antibody
    • eIF3i antibody
    • EIF3I_HUMAN antibody
    • EIF3S2 antibody
    • EIF3S2, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit 2 antibody
    • Eukaryotic translation initiation factor 3 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 3 subunit I antibody
    • eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD antibody
    • eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa antibody
    • eukaryotic translation initiation factor 3, subunit 2, formerly antibody
    • eukaryotic translation initiation factor 3, subunit I antibody
    • Predicted protein of HQ2242 antibody
    • PRO2242 antibody
    • TGF beta receptor interacting protein 1 antibody
    • TGF-beta receptor-interacting protein 1 antibody
    • TGFbeta receptor interacting protein 1 antibody
    • TGFbeta receptor-interacting protein 1 antibody
    • TRIP 1 antibody
    • TRIP-1 antibody
    • TRIP1 antibody
    see all


  • All lanes : Anti-EIF3S2 antibody (ab40745) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab40745 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40745, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of ab40745 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40745, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Marrugal Á  et al. Impact of Heat Shock Protein 90 Inhibition on the Proteomic Profile of Lung Adenocarcinoma as Measured by Two-Dimensional Electrophoresis Coupled with Mass Spectrometry. Cells 8:N/A (2019). Read more (PubMed: 31370342) »
  • Wang C  et al. Clusterin facilitates metastasis by EIF3I/Akt/MMP13 signaling in hepatocellular carcinoma. Oncotarget 6:2903-16 (2015). WB, IHC-P ; Human . Read more (PubMed: 25609201) »
See all 2 Publications for this product

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