Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF4A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 69-284 of Human eIF4A1.

  • Positive control

    • A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab137556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.

Target

  • Function

    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • Sequence similarities

    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP-dependent RNA helicase eIF4A-1 antibody
    • DDX2 antibody
    • DDX2A antibody
    • eIF 4A I antibody
    • eIF-4A-I antibody
    • EIF4A antibody
    • eIF4A I antibody
    • eIF4A-I antibody
    • EIF4A1 antibody
    • Eukaryotic initiation factor 4A-I antibody
    • Eukaryotic initiation factor 4AI antibody
    • eukaryotic translation initiation factor 4A, isoform 1 antibody
    • Eukaryotic translation initiation factor 4A1 antibody
    • IF4A1_HUMAN antibody
    see all

Images

  • Anti-eIF4A1 antibody (ab137556) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling eIF4A1 with ab137556 at 1/200 dilution. Lower image is costained with Hoechst 33342.

References

ab137556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for submitting your Abreview using ab137556 in rat samples in WB.
We haven't tested this antibody before with rat samples, so than you for providing these data. It is interesting that you are seeing a doublet in the WB. Do you think the upper band could correspond to a post-translationally modified version of the protein? Do you recall what you had used as a lysis buffer?
Thank you for the extra details. We appreciate your feedback!

Read More
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes soluble cell extracts)
Loading amount
15 µg
Specification
Cardiomyocytes soluble cell extracts
Gel Running Conditions
Reduced Denaturing (10% tris-glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Dec 13 2012

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