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    eif4a1-antibody-ab31217.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-eIF4A1 antibody (ab31217)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (39)

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Western blot - Anti-eIF4A1 antibody (ab31217)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (ab31217)
  • Western blot - Anti-eIF4A1 antibody (ab31217)
  • Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)

Key features and details

  • Rabbit polyclonal to eIF4A1
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-eIF4A1 antibody
    See all eIF4A1 primary antibodies
  • Description

    Rabbit polyclonal to eIF4A1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human eIF4A1 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab31796)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) This antibody gave a positive signal in the following tissue lysates: Liver (Mouse) Pancreas (Mouse) Testis (Mouse)
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human eIF4A1 peptide (ab31796)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IP
Human
WB
Mouse
Human
All applications
Cow
Monkey
African green monkey
Application Abreviews Notes
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IP (1)
Use at an assay dependent concentration.
ICC/IF (1)
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • Sequence similarities

    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Target information above from: UniProt accession P60842 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 504958 Cow
    • Entrez Gene: 1973 Human
    • Entrez Gene: 13681 Mouse
    • Entrez Gene: 287436 Rat
    • Omim: 602641 Human
    • SwissProt: Q3SZ54 Cow
    • SwissProt: P60842 Human
    • SwissProt: P60843 Mouse
    • Unigene: 129673 Human
    • Unigene: 279821 Mouse
    • Unigene: 371557 Mouse
    • Unigene: 91523 Rat
    see all
  • Alternative names

    • ATP-dependent RNA helicase eIF4A-1 antibody
    • DDX2 antibody
    • DDX2A antibody
    • eIF 4A I antibody
    • eIF-4A-I antibody
    • EIF4A antibody
    • eIF4A I antibody
    • eIF4A-I antibody
    • EIF4A1 antibody
    • Eukaryotic initiation factor 4A-I antibody
    • Eukaryotic initiation factor 4AI antibody
    • eukaryotic translation initiation factor 4A, isoform 1 antibody
    • Eukaryotic translation initiation factor 4A1 antibody
    • IF4A1_HUMAN antibody
    see all

Images

  • Western blot - Anti-eIF4A1 antibody (ab31217)
    Western blot - Anti-eIF4A1 antibody (ab31217)
    All lanes : Anti-eIF4A1 antibody (ab31217) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A-431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (ab31217)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (ab31217)
    ICC/IF image of ab31217 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31217, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence.  5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Western blot - Anti-eIF4A1 antibody (ab31217)
    Western blot - Anti-eIF4A1 antibody (ab31217)
    All lanes : Anti-eIF4A1 antibody (ab31217) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Mouse pancreas tissue lysate - total protein (ab29363)
    Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)
    Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)
    eIF4A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF4A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31217.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 47kDa: eIF4A1

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (39)

    Publishing research using ab31217? Please let us know so that we can cite the reference in this datasheet.

    ab31217 has been referenced in 39 publications.

    • Chu J  et al. Rocaglates Induce Gain-of-Function Alterations to eIF4A and eIF4F. Cell Rep 30:2481-2488.e5 (2020). PubMed: 32101697
    • Zhao Y  et al. USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair. Front Cell Dev Biol 8:529 (2020). PubMed: 32671073
    • Lee JS  et al. Loss of SLC25A11 causes suppression of NSCLC and melanoma tumor formation. EBioMedicine 40:184-197 (2019). PubMed: 30686754
    • Ryu I  et al. eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle. Cell Rep 26:2126-2139.e9 (2019). PubMed: 30784594
    • Hao Y  et al. Targetome analysis of chaperone-mediated autophagy in cancer cells. Autophagy N/A:1-14 (2019). PubMed: 30821613
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-eIF4A1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    mouse embryonic stem cells (J1)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 01 2018

    Western blot abreview for Anti-eIF4A1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse neuroblastoma N2A cells)
    Loading amount
    30 µg
    Specification
    Mouse neuroblastoma N2A cells
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 03 2012

    Western blot abreview for Anti-eIF4A1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLA)
    Loading amount
    20 µg
    Specification
    HeLA
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2012

    Immunoprecipitation abreview for Anti-eIF4A1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - other (SW480)
    Total protein in input
    30 µg
    Specification
    SW480
    Immuno-precipitation step
    Protein G
    Read More

    Abcam user community

    Verified customer

    Submitted May 24 2012

    Western blot abreview for Anti-eIF4A1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (Vero Cells)
    Loading amount
    10 µg
    Specification
    Vero Cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 24 2007

    Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4A1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% NP40
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 23 2007

    Question

    I would like to have informations about your eIF4A1 antibody (ab 31217). I use the sea urchin embryos for my analysis. I would like to know the sequence similarity and identity between the sea urchin eIF4A and the peptide that you used to produce this antibody. I can give you the sea urchin eIF4A sequence: MSYIDDEQANSQVTKPGPGPGAGPDENGRQAEDQGIVTVEDGIIDTNWDQVVENFDDMGLRESLLRGIYAYGFEKPSAIQQRAILPCCEGKDVIAQAQSGTGKTATFAISILQQLNVEEKQCQALVLAPTRELAQQIHKVIVALGDYLEVKCHPCIGGTNVRDDMNLLSEGVQVIVGTPGRVFDMLKRKVLSPFKIRMFVLDEADEMLSRGFKDQIYDVFSWLPSEVQVVLLSATMPVDVLEVTKKFMRDPIRILVKKEELSLDGIKQFYIAVEKEASLTGNGDERDTKRERGGWESIFIEDGLLLQSKSEVFEHGDMDQKERDKIMKEFRTGSSRVLICTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVPDEDKRNLRDIQQFYNTQVDEMPSNVADLV Can you tell me your opinion about the probability of cross reaction in sea urchin? Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2006

    Answer

    Thank you for your enquiry. Unfortunately it appears there is no significant homology between the immunogen and the seuence you provided. It is unlikely this antibody will cross-react with sea urchin. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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