Overview

  • Product name

  • Description

    Rabbit polyclonal to eIF4A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human eIF4A2 (internal sequence). The exact sequence is proprietary.
    Database link: Q14240

  • Positive control

    • WB: HeLa and HepG2 whole cell extracts. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-eIF4A2 antibody (ab228693) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 46 kDa



    10% SDS-PAGE gel.

  • Methanol-fixed A431 (Human epidermoid carcinoma cell line) cells stained for eIF4A2 (green) using ab228693 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue)

  • Paraffin-embedded human ovarian carcinoma tissue stained for eIF4A2 using ab228693 at 1/500 dilution in immunohistochemical analysis.

References

ab228693 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228693.
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