Key features and details
- Rabbit polyclonal to eIF4A2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-eIF4A2 antibody
See all eIF4A2 primary antibodies
DescriptionRabbit polyclonal to eIF4A2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Rhesus monkey
Recombinant fragment within Human eIF4A2 (internal sequence). The exact sequence is proprietary.
Database link: Q14240
- WB: HeLa and HepG2 whole cell extracts. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/20000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Sequence similaritiesBelongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- ATP dependent RNA helicase eIF4A 2 antibody
- ATP-dependent RNA helicase eIF4A-2 antibody
- BM-010 antibody
All lanes : Anti-eIF4A2 antibody (ab228693) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
10% SDS-PAGE gel.
Methanol-fixed A431 (Human epidermoid carcinoma cell line) cells stained for eIF4A2 (green) using ab228693 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue)
Paraffin-embedded human ovarian carcinoma tissue stained for eIF4A2 using ab228693 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228693 has not yet been referenced specifically in any publications.